Sepharose

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Sepharose is a tradename for a crosslinked, beaded-form of agarose, a polysaccharide polymer material extracted from seaweed. Its brand name is derived from Separation-Pharmacia-Agarose. A common application for the material is in chromatographic separations of biomolecules.

Sepharose is a registered trademark of GE Healthcare (formerly: Pharmacia, Pharmacia LKB Biotechnology, Pharmacia Biotech, Amersham Pharmacia Biotech, and Amersham Biosciences).

Various grades and chemistries of sepharose are available.[1] Iodoacetyl functional groups can be added to selectively bind cysteine side chains and this method is often used to immobilize peptides. Sepharose/agarose, combined with some form of activation chemistry, is also used to immobilize enzymes, antibodies and other proteins and peptides through covalent attachment to the resin.[2] Common activation chemistries include cyanogen bromide (CNBr) activation and reductive amination of aldehydes to attach proteins to the agarose resin through lysine side chains.

See also[edit]

Gel permeation chromatography

References[edit]

  1. ^ Ahmed, Hafiz (2004). Principles and Reactions of Protein Extraction, Purification, and Characterization. CRC Press. p. 143. 
  2. ^ "Immobilized Nucleotide and Nucleic acid Affinity". GE Healthcare Life Sciences US Site. Retrieved 20 Jan 2015.