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She graduated law school in 1907 with a thesis, "Humanism and Feminism", that questioned the submission of women, argued for the education of women and the improvement of women's status in marriage, and was controversial. She also founded with Virginia Corvalán and others the Feminist Movement for Assumption (in 1919), the Paraguayan Feminist Center, the Feminine Union of Paraguay, and the Paraguayan League for the Rights of Women. In 1904 she and twenty other women founded the Committee of Women for Peace.
She earned her teaching diploma from the Normal School for Female Teachers in 1898, earned her bachelor's degree from the Colegio National in 1902, and worked as a professor at the National College of the Capital starting in 1904; she founded (possibly with others) the Mercantile School for Girls (a business school) in 1905. In the first International Feminist Congress in 1910 in Argentina, Dávalos was the official delegate of Paraguay, and was appointed as a member of the Executive Committee of the Pan-American Women's Federation. She was later selected as Honorary President of the National Council of Women of Paraguay.
In 1936 she was the Consul to the Paraguay Feminine Union (UFP). In 1952, she participated in the Paraguayan League for the Rights of Women.
She never married and lived with "[h]er constant companion, Honoria Ballirán", as mentioned in 1959 by Dr. Ignacio Amado Berino, Secretary General of the National University of Asunción, in his lecture on "Serafina Dávalos, the precursor of feminism in Paraguay," published in the newspaper "El Feminista" and reproduced as an annex to the thesis of "Serafina Dávalos: Humanismo,” conducted by the Centre for Documentation and Studies and RP Ediciones.
In 1998, long after her death, she became the first woman to appear on a Paraguayan postage stamp when the General Post Office of Paraguay issued stamps that read "The First Female Lawyer and Feminist of Paraguay (1883–1957)." There is also a street named in her honor in the administrative center of Coronel Oviedo, the city where she was born.
- "Serafina Dávalos". 1325mujerestejiendolapaz.org. Retrieved 2013-10-29.
- "Famous people from Paraguay, famous natives sons". Worldatlas.com. Retrieved 2013-10-29.
- Gender, Citizenship, and Local Democracy in Paraguay: A Comparative Analysis ... – Jane Clough-Riquelme – Google Books. Books.google.com. Retrieved 2013-10-29.
- Paraguayans of To-day – Google Books. Books.google.com. Retrieved 2013-10-29.
- "Same-Sex Marriage in Argentina – A Reflection on the Institution of Marriage | International Gay and Lesbian Human Rights Commission". Iglhrc.wordpress.com. Retrieved 2013-10-29.