Sex-limited genes are genes that are present in both sexes of sexually reproducing species but are expressed in only one sex and remain 'turned off' in the other. In other words, sex-limited genes cause the two sexes to show different traits or phenotypes, despite having the same genotype. This term is restricted to autosomal traits, and should not be confused with sex-linked characteristics, which have to do with genetic differences on the sex chromosomes (see sex-determination system). Sex-limited genes are also distinguished from sex-influenced genes, where the same gene will show differential expression in each sex. Sex-influenced genes commonly show a dominant/recessive relationship, where the same gene will have a dominant effect in one sex and a recessive effect in the other (for example, male pattern baldness).
Sex-limited genes are responsible for sexual dimorphism, which is a phenotypic (directly observable) difference between males and females of the same species. These differences can be reflected in size, color, behavior (ex: levels of aggression), and morphology. An example of sex-limited genes are genes which instruct the male elephant seals to grow big and fight, at the same time instructing female seals to grow small and avoid fights. These genes are also responsible for some female beetles' inability to grow exaggerated mandibles, research that is discussed in detail later in this article.
The overall point of sex-limited genes is to resolve intralocus sexual conflict. In other words, these genes try to resolve the "push-pull" between males and females over trait values for optimal phenotype. Without these genes, organisms would be forced to settle on an average trait value, incurring costs on both sexes. With these genes, it is possible to 'turn off' the genes in one sex, allowing both sexes to attain (or at least, approach very closely) their optimal phenotypes.
A brief history
Unsurprisingly, the idea of sex-limited genes was initially developed by Charles Darwin himself in 1871 in his book The Descent of Man and Selection in Relation to Sex. He does not distinguish between sex-limited, sex-linked, and sex-influenced genes, but refers to any gene that expresses differently between sexes as sex-limited. While this concept was still in its infancy, Darwin catalyzed the further development of sex-related selection. Thomas Hunt Morgan, fully aware of this confusing terminology, published an article in The American Naturalist in 1914 titled "Sex-Linked and Sex-Limited Inheritance." This article directly acknowledges that Darwin applied the term sex-limited whenever a characteristic seemed specific to one sex. Morgan proposes the definitions for sex-linked genes and sex-limited genes that we still use today (and that were defined in the introduction above). This paper helped to distinguish between these two similar concepts and clarify much confusion in the scientific community at the time. Morgan's paper was followed by several others involving sex-limited genes and their expression as traits. One of the more notable examples is John H. Gerould's "Inheritance of White Wing Color, a Sex-Limited (Sex-Controlled) Variation in Yellow Pierid Butterflies," published in Genetics in 1923 (and edited slightly in 1924). Gerould observed that in this species of butterfly, females naturally occur as yellow or white, while males only occur with yellow coloration. He extensively explores this apparently sex-limited trait from a genetic perspective in this ground-breaking 50 page paper. To conclude the notable advancements in the early stages of the development of sex-limited genes, a brief discussion of R. A. Fisher is necessary. Commonly hailed as one of the best evolutionary biologists of his time, Fisher was also a talented geneticist. His book The Genetical Theory of Natural Selection, published in 1930, over 20 years before the double-helix shape of DNA was discovered, was the first attempt to explain Darwin's theories within the foundation of genetics. Chapter 6 of this book is titled "Sexual Reproduction and Sexual Selection" and includes a genetic interpretation of Darwin's initial idea of sex-limited genes. After these groundbreaking works, papers continue to be published further exploring the causes, mechanisms, evolutionary advantages, and more of sex-limited genes.
Many studies have been published exploring the genetic basis of sex-limited genes. One paper, published in Evolution, evaluates the hypothesis that sex-limited traits can arise in two ways. The alleles responsible for sexual dimorphism can be limited to expression in only one sex when they first appear, or the alleles could begin by being expressed in both sexes then become modified (repressed or promoted) in one sex by modifier genes or regulatory elements. The concept of this study was to examine female hybrids from species where males displayed different types of ornamental traits (elongated feathers, wattles, color patches). The assumption is that different hypotheses about male-specific expression will yield different results in female hybrids. The methods and materials of the experiment are discussed in detail in the paper, but the important result that emerged was that NO female hybrids expressed any of the ornamental traits found in the parent males. Two interpretations of these results are possible: the dimorphic alleles were initially only expressed in males, or the alleles were initially expressed in both and then were suppressed in females or became limited to males by regulatory regions that are completely dominant in hybrids. The most likely genomic explanation for initial expression in both species then modification is involvement of cis-dominance, where the factors that modify the gene are located next to the gene on the chromosome. (This is in contrast to trans-dominance, where mobile products that can affect distant genes are produced.) These factors can be in the form of promoter regions, which can be either be suppressed or activated by hormones. This experiment also demonstrates that these alleles come under regulatory control very quickly. This is because none of the ornamentation seen in males is seen in the very next generation. These conclusions make it likely that at least some male-specific (thus, sex-limited) genes cue their expression by hormone levels - the absence of estrogen or the presence of testosterone.
Because sex-limited genes are present in both sexes but only expressed in one, this allows the unexpressed genes to be hidden from selection. On a short-term scale, this means that during one generation, only the sex that expresses the sex-limited trait(s) of interest will be affected by selection. The remaining half of the gene pool for these traits will be unaffected by selection because they are hidden (unexpressed) in the genes of the other sex. Since a portion of the alleles for these sex-limited traits are hidden from selection, this occurrence has been termed 'storage-effect'. On a long-term scale, this storage effect can have significant impacts on selection, especially if selection is fluctuating over a long period of time. It is inarguable that selection will fluctuate over time with varying levels of environmental stability. For example, fluctuations in population density can drive selection on sex-limited traits. In less dense populations, females will have less opportunity to choose between males for reproduction. In this case, attractive males may experience both reduced reproductive success and increased predation pressure. Thus, selection on males for sex-limited traits such as increased size (elephant seals) and weaponry (claws on fiddler crabs, horns on rhinoceros beetles) will change direction with fluctuation in population density.
John Parsch and Hans Ellegren defined "genes that differ in expression between females and males" as sex-biased genes. While this definition is more broad, sex-limited genes are certainly included in this category. One of the key principles of sex-biased gene expression that Parsch and Ellegren stressed in their paper in February 2013 is that of rapid evolution. They assert that a gene's sex bias can vary among different types of tissues throughout the body or throughout development, making the level of sex bias a fluid, rather than static, property. This makes it possible, then, that the rapid evolution seen in sex-biased genes is not an inherent property of their sex bias, but a property of some other feature. The paper offers expression breadth, the number of tissue types in which the genes are expressed, as an example of a feature correlated to sex-biased genes. It is known that genes with limited expression (in only one type of tissue) generally evolve faster than those with a higher expression breadth, and sex-biased genes are often restricted in their expression, such as to only the testes or ovaries. Thus, it is likely that sex-biased (including sex-limited) genes will evolve faster than the average genetic information. Parsch and Ellegren also assert that "sex-biased genes expressed only in sex-limited reproductive tissues evolve faster than unbiased genes that are expressed only in a single, non-reproductive tissue." That is, genes that have a bias toward any kind of reproductive tissue (testes or ovaries) seem to show faster evolution than genes expressed in non-gonadal tissues, despite the number of tissues in which they are expressed. This makes sense in the context of genes with reproductive function evolving more quickly, a generally observed pattern in evolutionary biology.
Effects of sexual antagonism
Sexual antagonism occurs when two species have conflicting optimal fitness strategies concerning reproduction (see link in introduction paragraph). Multiple matings is a classic example of competing optimal strategies. Males, who typically have a much lower overall investment in reproduction, may benefit from more frequent matings. Females, however, invest much more in reproduction and can be endangered, harmed, or even killed by multiple matings.
In 2010, Hosken et al. completed an important study exploring the effects of sexually antagonistic selection on sex-limited trait expression. They asked if sex-specific trait selection always resolved intralocus conflict, as it was believed to do. By using a species of flour beetle, Gnatocerus cornutus, exhibiting sex-limited traits in the form of exaggerated mandible size, they were able to test this hypothesis. Exaggerated mandibles are only developed in males; females never develop exaggerated mandibles. The point of this experiment was to determine how mandibles affect fitness. If these sex-limited genes are truly quelling intralocus sexual conflict, male mandible size should have no effect on female fitness. After selecting for males with exaggerated mandibles (full materials and methods can be found within the paper), it was experimentally determined that males with exaggerated mandibles had a higher fitness - they experienced increased fighting and mating success. It was also found, however, that females found in the populations of males with exaggerated mandibles had lower fitness (as determined by lifetime reproductive success, LRS) relative to the fitness of females in populations with males with smaller mandibles. Since this male sex-limited trait has an impact on female fitness, intralocus sexual conflict has not been resolved. This highlights the importance of sexual conflict to evolution, because it cannot simply be defused by sex-limited trait expression.
Later the same year, a paper in Evolution also came to the same conclusions about sexual antagonism in relation to sex-limited genes. They developed a mathematical model to show that the fitness costs of sexual antagonism, even when rare, will usually overwhelm the benefits of sexually concordant selection. (Sexually concordant selection occurs when selection favors the same alleles in both sexes but differs in relative strength between them.) Through several advanced calculations, they concluded that even a small relative amount of sexual antagonism will overwhelm any benefit harvested from sexually concordant selection. Coming to the same conclusion as Hosken et al., they demonstrated mathematically that when sex-limited gene expression attempts to resolve sexual antagonism, it is likely to produce negative long-term fitness consequences. This result is seen in the experiment with beetles above, where the females demonstrate reduced fitness in response to males selected for larger mandibles. So, with mathematical support and a lack of support for strong fitness benefits as a result of sexually concordant selection, the paper concludes that sex-specific selection is more likely to incur costs than benefits to sexually reproducing species.
Effects on animal behavior
Animal behavior (see ethology) encompasses so many disciplines that it is impossible not to see it in some capacity in almost all primary literature involving live animals. While the examples above certainly contain aspects of animal behavior, a more overt example of it in relation to sex-limited traits is detailed in a Teplitsky et al. paper (2010) centering around breeding time in red-billed gulls. This experiment deals with breeding time, an aspect of reproductive biology. Reproduction and sexual behavior are two key aspects of animal behavior, as they are universally expressed in some way throughout the animal kingdom.
Breeding time in red-billed gulls is expressed only in females, because only females lay eggs. Male care, however, affects female breeding performance substantially. This qualifies breeding time as a sex-limited trait because it is expressed only in one sex but can be affected by both (similarly to Hosken's beetle experiment above). By following a natural population of red-billed gulls for 46 years, Teplitsky et al. came to an unexpected conclusion - while laying date (aka breeding time) is only expressed in females, the trait is only heritable in males. This is atypical because sex-limited traits are almost always heritable within the sex in which they are expressed.
For this species, the timing of egg-laying has much to do with male behavior. Males can affect female reproductive success so strongly because for the 20 days up to egg-laying, females spend up to 80% of their time in the nest. This leaves males with the responsibility of providing food regularly and securing (and maintaining) a high-quality territory for nesting. This phenomenon of the genetics of one individual affecting those of another individual is known as indirect genetic effects. For this population, at least, possible explanations for this atypical heritability pattern exist. While controlling female health and safety, males are responsible for the timing of the start of courtship feeding, as well. These populations also typically have excesses of females, allowing males to exert even further choice in the form of mate choice. These factors in combination give males a great opportunity to express their "laying date genotype". In spite of the presence of directional selection and significant male heritability for breeding time, no advancement of breeding time was seen during the 46 years of this experiment. This does not discount the significance of the paper's other results however - one of the most significant being that here a "female trait (laying date) is largely determined by genetic characteristics of its mate".
Overall, sex-limited genes carry with them several complex cost to benefit ratios which call for further analysis. For example, while they allow for greater opportunities of sexual dimorphism so both sexes can reach much closer to their optimal phenotypes, they also incur fitness costs on sexually reproducing species. Since Charles Darwin's revolutionary book was published in 1871, there have been many studies done on the nature of these genes. The scientific literature dealing with the concepts of sexual dimorphism and sex-limited genes extends far past what has been listed here. The genetic and mechanistic details of these genes are still being discovered through ongoing research today. It is becoming apparent that a deeper understanding sex-limited genes will be increasingly important as the fields of evolutionary biology and genetics advance.
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- Hosken, D.J.; et al. (2012). September 2013 "Intralocus Sexual Conflict Unresolved By Sex-Limited Trait Expression". Current Biology 20 (22): 2036–2039. Retrieved 22.
- Darwin, Charles (1871). The Descent of Man and Selection in Relation to Sex. New York: A. L. Burt.
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- Gerould, John H. (November 1923). "Inheritance of White Wing Color, a Sex-Limited (Sex-Controlled) Variation in Yellow Pierid Butterflies". Genetics 8 (6): 495–551.
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