Sf9 (cells)

Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-SF21-AE), are commonly used for recombinant protein production using baculovirus. They were originally established from ovarian tissue.^[1] They can be grown in the absence of serum, and can be cultured attached or in suspension.^[2]

References

^ Vaughn JL, Goodwin RH, Tompkins GJ, McCawley P (1977). "The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae)". In Vitro. 13 (4): 213–217. doi:10.1007/BF02615077. PMID 68913.{{cite journal}}: CS1 maint: multiple names: authors list (link)
^ Invitrogen (03/08/2010). "Cell Lines". Growth and Maintenance of Insect cell lines (PDF). Rev. Date - 8 March 2010. Invitrogen. Manual part no. 25-0127, MAN0000030. Retrieved 2012-02-23. {{cite book}}: Check date values in: |date= (help); Cite has empty unknown parameter: |sectionurl= (help)

External links

Cellosaurus entry for Sf9

[1] Vaughn JL, Goodwin RH, Tompkins GJ, McCawley P (1977). "The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae)". In Vitro. 13 (4): 213–217. doi:10.1007/BF02615077. PMID 68913.{{cite journal}}: CS1 maint: multiple names: authors list (link)

[2] Invitrogen (03/08/2010). "Cell Lines". Growth and Maintenance of Insect cell lines (PDF). Rev. Date - 8 March 2010. Invitrogen. Manual part no. 25-0127, MAN0000030. Retrieved 2012-02-23. {{cite book}}: Check date values in: |date= (help); Cite has empty unknown parameter: |sectionurl= (help)

[1]

[2]