Barbiro graduated from Beit Zvi Actors School of Performing Arts in 1991.
He is known for his voice-over work in animated TV shows and feature films. Some of his prominent work includes the Hebrew dubs for Stitch and Dr. Hamsterviel in Lilo & Stitch: The Series and its anime television adaptation, Mort from the Madagascar series, Harvey Bullock from Batman: The Animated Series, Krillin and Frieza from Dragon Ball ("Z" and "GT").
Following the death of Yoni Chen in 1995, Barbiro replaced him in Looney Tunes feature film, Bugs Bunny's 3rd Movie: 1001 Rabbit Tales as Elmer Fudd (until the first DVD edition dub) and Sylvester (until The Sylvester & Tweety Mysteries). Both roles were passed on to Ami Mendelman.
Following Giora Kenneth's retirement from voice work in 2007, due to health problems in his throat, Barbiro replaced him as Mr. Herriman in Foster's Home for Imaginary Friends and Minimus P.U. in Atomic Betty.
He is a close friend of Yoram Yosefsberg who co-played Allot in dubbing works.
As an actor, Barbiro has participated in TV shows, radio commercials and plays.
- Bat-Sheva veSimcha Holchim leBroadway (Bat-Sheva and Simcha Go to Broadway) with Bat-Sheva Zeisler and Bart Berman
- Ivanov with the Cameri Theater (Dmitry Kosykh)
- Ha'ee (A guy in the kiosk at the Marine)
- LaLa Land (Gershon)
- Mendelbaum Balash Prati (Jewelry Store Owner)
- Polishuk (Israel Bajonder)
- Shemesh (Menahem)
- "Profile of Simacha Barbiro at Beit-Zvi" (Hebrew)
- Lev-Ari, Shimon (June 2014), "צייזלר בת-שבע", מדריך 100 שנה לתאטרון העברי 1889-1989, Tel Aviv University, retrieved 29 March 2015,
שיחקה בהצגות: את ואני והמלחמה הבאה, כל מה שרצית לדעת על אופן ולא העזת לשאול את באך, אופרת הקבצנים, אנדרטה הפוכה, אינטימיות, גבירתי הנאוה, החולה המדומה, כוכב הבכי, בת שבע ושמחה הולכים לברודווי, כוכב הבכי ועוד.
- Kaye, Helen (11 January 2015). "Theater Review: Ivanov". The Jerusalem Post. p. 24. Retrieved 29 March 2015.
The two harpies, Kaplan’s Zuzushka and Ben-Yehuda’s Marfusha gloriously provide more comic relief, as does Simcha Barbiro as petty-thief and sponger Kossich. [sic: Kosykh]