Sirius Red

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Sirius Red
Sirius Red F 3B.svg
IUPAC name
Hexasodium (3E)-4-oxo-7-[[(6E)-5-oxo-7-sulfonato-6-[[2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl]hydrazinylidene]naphthalen-2-yl]carbamoylamino]-3-[[2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl]hydrazinylidene]naphthalene-2-sulfonate
Other names
Direct Red 80; Picrosirius Red
3D model (JSmol)
Molar mass 1,373.05 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Sirius Red F 3B (Direct Red 80) is a azo dye used principally in staining methods for collagen and amyloid with the molecular formula C45H26N10Na6O21S6.[1]

In histology, sirius red staining is used in various domains of diagnostic to observe fibrosis levels in a lot of cases of inflammation induced by cancer, vascular or metabolic pathologies.[2]

In bright field microscopy the following can be observed:

  • The nuclei in yellow
  • The cytoplasm in yellow
  • Collagen fibers in red
  • Muscular fibers in yellow
  • Red blood cells in yellow


  1. ^ ResearchGate"Certification procedures for sirius red F3B". Retrieved 2015-08-21.
  2. ^ Sirius red