A sister chromatid refers to either of the two identical copies (chromatids) formed by the replication of a single chromosome, with both copies joined together by a common centromere. In other words, a sister chromatid may also be said as 'one-half' of the duplicated chromosome. A full set of sister chromatids is created during the synthesis (S) phase of interphase, when all the chromosomes in a cell are replicated. The two sister chromatids are separated from each other into two different cells during mitosis and during the second division of meiosis straight after fertilization during sexual reproduction.
Compare sister chromatids to homologous chromosomes, which are the two different copies of a chromosome that diploid organisms (like humans) inherit, one from each parent. Sister chromatids are by and large identical (since they carry the same alleles, also called variants or versions, of genes) because they derive from one original chromosome. An exception is towards the end of meiosis, after crossing over has occurred, because sections of each sister chromatid may have been exchanged with corresponding sections of the homologous chromatids with which they are paired during meiosis. Homologous chromosomes might or might not be the same as each other because they derive from different parents.
There is evidence that, in some species, sister chromatids are the preferred template for DNA repair. Sister chromatid cohesion is essential for the correct distribution of genetic information between daughter cells and the repair of damaged chromosomes. Defects in this process may lead to aneuploidy and cancer, especially when checkpoints fail to detect DNA damage or when incorrectly attached mitotic spindles don't function properly.
Mitotic recombination is primarily a result of DNA repair processes responding to spontaneous or induced damages. (Also reviewed in Bernstein and Bernstein, pp 220–221 Homologous recombinational repair during mitosis is largely limited to interaction between nearby sister chromatids that are present in a cell subsequent to DNA replication but prior to cell division. Due to the special nearby relationship they share, sister chromatids are not only preferred over distant homologous chromatids as substrates for recominational repair, but have the capacity to repair more DNA damage than do homologs.
Studies with the budding yeast Saccharomyces cerevisiae indicate that inter-sister recombination occurs frequently during meiosis, and up to one-third of all recombination events occur between sister chromatids.
- Kadyk, Lc; Hartwell, Lh (Oct 1992). "Sister chromatids are preferred over homologs as substrates for recombinational repair in Saccharomyces cerevisiae" (Free full text). Genetics 132 (2): 387–402. ISSN 0016-6731. PMC 1205144. PMID 1427035.
- Moynahan ME, Jasin M (2010). "Mitotic homologous recombination maintains genomic stability and suppresses tumorigenesis". Nat. Rev. Mol. Cell Biol. 11 (3): 196–207. doi:10.1038/nrm2851. PMC 3261768. PMID 20177395.
- Symington LS, Rothstein R, Lisby M (2014). "Mechanisms and regulation of mitotic recombination in Saccharomyces cerevisiae". Genetics 198 (3): 795–835. doi:10.1534/genetics.114.166140. PMC 4224172. PMID 25381364.
- Bernstein C, Bernstein H. (1991) Aging, Sex, and DNA Repair. Academic Press, San Diego. ISBN 978-0120928606 partly available at http://books.google.com/books?id=BaXYYUXy71cC&pg=PA3&lpg=PA3&dq=Aging,+Sex,+and+DNA+Repair&source=bl&ots=9E6VrRl7fJ&sig=kqUROJfBM6EZZeIrkuEFygsVVpo&hl=en&sa=X&ei=z8BqUpi7D4KQiALC54Ew&ved=0CFUQ6AEwBg#v=onepage&q=Aging%2C%20Sex%2C%20and%20DNA%20Repair&f=false
- Goldfarb T, Lichten M (2010). "Frequent and efficient use of the sister chromatid for DNA double-strand break repair during budding yeast meiosis". PLoS Biol. 8 (10): e1000520. doi:10.1371/journal.pbio.1000520. PMC 2957403. PMID 20976044.