Small Cajal body-specific RNA

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Small Cajal body specific RNA 1
RF00553.png
Predicted secondary structure of Small Cajal body specific RNA 1
Identifiers
Symbol SCARNA1
Rfam RF00553
Other data
RNA type gene, snRNA, snoRNA, scaRNA;
Domain(s) Vertebrata

Small Cajal body-specific RNAs (scaRNAs) are a class of small nucleolar RNAs (snoRNAs) that specifically localise to the Cajal body, a nuclear organelle (cellular sub-organelle) involved in the biogenesis of small nuclear ribonucleoproteins (snRNPs or snurps). ScaRNAs guide the modification (methylation and pseudouridylation) of RNA polymerase II transcribed spliceosomal RNAs U1, U2, U4, U5 and U12.

The first scaRNA identified was U85.[1] It is unlike typical snoRNAs in that it is a composite C/D box and H/ACA box snoRNAs and can guide both pseudouridylation and 2'-O-methylation. Not all scaRNAs are composite C/D and H/ACA box snoRNA and most scaRNAs are structurally and functionally indistinguishable from snoRNAs, directing ribosomal RNA (rRNA) modification in the nucleolus.

Drosophila scaRNAs[edit]

Several studies identified scaRNAs in Drosophila. [1][2][3][4][5] One of the studies showed that several Drosophila scaRNAs could function equally well in the nucleoplasm of mutant flies that lack Cajal bodies.[4] Further investigation showed that scaRNA pugU6-40 targets U6 snRNA, whose modification occurs in the nucleolus not CB and that pugU1-6 and pug U2-55 guide 2 RNAs: snRNA and 28s rRNA.[5]

Small Cajal body-specific RNA 1[edit]

In molecular biology, Small Cajal body-specific RNA 1 (also known as SCARNA1 or ACA35) is a small nucleolar RNA found in Cajal bodies and believed to be involved in the pseudouridylation of U2 spliceosomal RNA at residue U89.

scaRNA1 is a non-coding RNA, which are functional products of genes not translated into proteins. Such RNA molecules usually contain important secondary structure or ligand-binding motifs and are involved in many important biological processes in the cell.[6]

scaRNA1 belongs to the H/ACA box class of snoRNAs, as it has the predicted hairpin-hinge-hairpin-tail structure, conserved H/ACA-box motifs, and is found associated with GAR1 protein.[7]

References[edit]

  1. ^ a b Jády BE, Kiss T (2001). "A small nucleolar guide RNA functions both in 2′-O-ribose methylation and pseudouridylation of the U5 spliceosomal RNA". EMBO J. 20 (3): 541–51. doi:10.1093/emboj/20.3.541. PMC 133463Freely accessible. PMID 11157760. 
  2. ^ Tortoriello, Giuseppe; Accardo, Maria Carmela; Scialò, Filippo; Angrisani, Alberto; Turano, Mimmo; Furia, Maria (2009-05-01). "A novel Drosophila antisense scaRNA with a predicted guide function". Gene. 436 (1-2): 56–65. doi:10.1016/j.gene.2009.02.005. ISSN 1879-0038. PMID 19230845. 
  3. ^ Tycowski, Kazimierz T.; Shu, Mei-Di; Kukoyi, Abiodun; Steitz, Joan A. (2009-04-10). "A conserved WD40 protein binds the Cajal body localization signal of scaRNP particles". Molecular Cell. 34 (1): 47–57. doi:10.1016/j.molcel.2009.02.020. ISSN 1097-4164. PMC 2700737Freely accessible. PMID 19285445. 
  4. ^ a b Deryusheva, Svetlana; Gall, Joseph G. (2009-12-01). "Small Cajal body-specific RNAs of Drosophila function in the absence of Cajal bodies". Molecular Biology of the Cell. 20 (24): 5250–5259. doi:10.1091/mbc.E09-09-0777. ISSN 1939-4586. PMC 2793299Freely accessible. PMID 19846657. 
  5. ^ a b Deryusheva, Svetlana; Gall, Joseph G. (2013-12-01). "Novel small Cajal-body-specific RNAs identified in Drosophila: probing guide RNA function". RNA (New York, N.Y.). 19 (12): 1802–1814. doi:10.1261/rna.042028.113. ISSN 1469-9001. PMC 3884663Freely accessible. PMID 24149844. 
  6. ^ Darzacq, X; Jady BE; Verheggen C; Kiss AM; Bertrand E; Kiss T (2002). "Cajal body-specific small nuclear RNAs: a novel class of 2′-O-methylation and pseudouridylation guide RNAs". EMBO J. 21 (11): 2746–2756. doi:10.1093/emboj/21.11.2746. PMC 126017Freely accessible. PMID 12032087. 
  7. ^ Kiss, Arnold M.; Jády, Beáta E.; Bertrand, Edouard; Kiss, Tamás (2004). "Human Box H/ACA Pseudouridylation Guide RNA Machinery". Molecular and Cellular Biology. 24 (13): 5797–5807. doi:10.1128/MCB.24.13.5797-5807.2004. PMC 480876Freely accessible. PMID 15199136. 

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