Small nucleolar RNA SNORD47

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Small nucleolar RNA SNORD47
RF00281.jpg
Identifiers
Symbol SNORD47
Alt. Symbols U47
Rfam RF00281
Other data
RNA type Gene; snRNA; snoRNA; C/D-box
Domain(s) Eukaryota
GO 0006396 0005730
SO 0000593

SNORD47 (also known as U47) is a non-coding RNA (ncRNA) molecule which functions in the modification of other small nuclear RNAs (snRNAs). This type of modifying RNA is usually located in the nucleolus of the eukaryotic cell which is a major site of snRNA biogenesis. It is known as a small nucleolar RNA (snoRNA) and also often referred to as a guide RNA.

snoRNA U57 belongs to the C/D box class of snoRNAs which contain the conserved sequence motifs known as the C box (UGAUGA) and the D box (CUGA). Most of the members of the box C/D family function in directing site-specific 2'-O-methylation of substrate RNAs.[1]

This snoRNA was originally cloned from HeLa cells and expression verified by northern blotting.[2] It is predicted to guide 2'O-ribose methylation of ribosomal RNA (rRNA) 28S at residue C3866. The mouse orthologue was also cloned[3]

This snoRNA is encoded in the introns of the same genes as other C/D box snoRNAs U44, U74, U75, U76, U77, U78, U79, U80 and U81.[4]

References[edit]

  1. ^ Galardi, S.; Fatica, A.; Bachi, A.; Scaloni, A.; Presutti, C.; Bozzoni, I. (October 2002). "Purified Box C/D snoRNPs Are Able to Reproduce Site-Specific 2'-O-Methylation of Target RNA in Vitro". Molecular and Cellular Biology. 22 (19): 6663–6668. doi:10.1128/MCB.22.19.6663-6668.2002. PMC 134041Freely accessible. PMID 12215523. 
  2. ^ Kiss-László, Zsuzsanna; Henry, Yves; Bachellerie, Jean-Pierre; Caizergues-Ferrer, Michèle; Kiss, Tamás (June 1996). "Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs". Cell. 85 (7): 1077–1088. doi:10.1016/S0092-8674(00)81308-2. PMID 8674114. 
  3. ^ Huttenhofer, Alexander; Kiefmann, Martin; Meier-Ewert, Sebastian; O'Brien, John; Lehrach, Hans; Bachellerie, Jean-Pierre; Brosius, Jürgen (1 June 2001). "RNomics: an experimental approach that identifies 201 candidates for novel, small, non-messenger RNAs in mouse". The EMBO Journal. 20 (11): 2943–2953. doi:10.1093/emboj/20.11.2943. PMC 125495Freely accessible. PMID 11387227. 
  4. ^ Lestrade, Laurent; Weber, Michel J. (2006). "snoRNA-LBME-db, a comprehensive database of human H/ACA and C/D box snoRNAs". Nucleic Acids Research. 34 (Supplement 1: Database Issue): D158–D162. CiteSeerX 10.1.1.105.7552Freely accessible. doi:10.1093/nar/gkj002. PMC 1347365Freely accessible. PMID 16381836. 

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