These describe how the population of the different spin states changes in relation to the strength of the self-relaxation rate constant R and , which accounts instead for cross-relaxation. The latter is the important term which is responsible for transferring magnetization from one spin to the other and gives rise to the nuclear Overhauser effect.
In an NOE experiment, the magnetization on one of the spins, say spin 2, is reversed by applying a selective pulse sequence. At short times then, the resulting magnetization on spin 1 will be given by
since there is no time for a significant change in the populations of the energy levels. Integrating with respect to time gives:
which results in an enhancement of the signal of spin 1 on the spectrum. Typically, another spectrum is recorded without applying the reversal of magnetization on spin 2 and the signals from the two experiments are then subtracted. In the final spectrum, only peaks which have an nOe enhancement show up, demonstrating which spins are in spatial proximity in the molecule under study: only those will in fact have a significant cross relaxation factor.
- Solomon, I (1955). "Relaxation Processes in a System of Two Spins" (PDF). Phys. Rev. 99: 559. doi:10.1103/PhysRev.99.559.
- Arthur Palmer. "Relaxation and Dynamic Processes" (PDF). Columbia University. Archived from the original (PDF) on 2014-05-08. Retrieved 2012-08-05.
- James Keeler (2010). Understanding NMR Spectroscopy (2nd ed.). Wiley. pp. 274–278. ISBN 9780470746080.