Studio One or Studio 1 may refer to:
- Westinghouse Studio One, an American radio–television anthology series 1948–1958
- Studio One (TV show), a television show in the MENA region, broadcast on Dubai One
- Studio One (New Zealand series), a TV show in New Zealand
- Studio One (record label), a Jamaican recording studio and record label in 1954–1980s
- Studio One (recording studio), an American facility in 1970–1989
- Studio 1 (album), a 2006 album by British girl group All Saints
- Studio One (company), an American marketing company
- Studio One (nightclub), (later The Factory), a nightclub in West Hollywood, United States
- Studio One 19, a computer from Dell, released in 2009
- PreSonus Studio One, audio recording software
- Disney Studio 1, a building in Disneyland Paris, France
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