Sulforhodamine B

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Sulforhodamine B
Sulforhodamine B
IUPAC name
3D model (JSmol)
ECHA InfoCard 100.020.482
EC Number 220-025-2
Molar mass 558.666 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Sulforhodamine B or Kiton Red 620 (C27H30N2O7S2) is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells. This red solid dye is very water-soluble.[1]


The dye has maximal absorbance at 565 nm light and maximal fluorescence emission at 586 nm light.[1] It does not exhibit pH-dependent absorption or fluorescence over the range of 3 to 10.[2]


Sulforhodamine B is often used as a membrane-impermeable polar tracer[3] or used for cell density determination via determination of cellular proteins (cytotoxicity assay).[4]


  1. ^ a b "Sulforhodamine B sodium salt (CAS 3520-42-1)". Santa Cruz Biotechnology. 
  2. ^ Coppeta, J.; Rogers, C. (1998). "Dual Emission Laser Induced Fluorescence for Direct Planar Scalar Behavior Measurements". Experiments in Fluids. 25: 1–15. doi:10.1007/s003480050202. 
  3. ^ Viricel W; Mbarek A; Leblond J (2015). "Switchable Lipids: Conformational Change for Fast pH-Triggered Cytoplasmic Delivery". Angewandte Chemie International Edition. 54: 12743–12747. PMID 26189870. doi:10.1002/anie.201504661. 
  4. ^ Vichai V; Kirtikara K (2006). "Sulforhodamine B colorimetric assay for cytotoxicity screening". Nature Protocols. 1: 1112–1116. doi:10.1038/nprot.2006.179.