Sulforhodamine B

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Sulforhodamine B
Sulforhodamine B
Names
IUPAC name
2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate
Identifiers
3520-42-1 YesY
ChemSpider 58690 YesY
EC Number 220-025-2
Jmol 3D model Interactive image
PubChem 65191
Properties
C27H30N2O7S2
Molar mass 558.666 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
YesY verify (what is YesYN ?)
Infobox references

Sulforhodamine B or Kiton Red 620 (C27H30N2O7S2) is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells. This red solid dye is very water-soluble.[1]

The dye has maximal absorbance at 565 nm light and maximal fluorescence emission at 586 nm light.[1] It does not exhibit pH-dependent absorption or fluorescence over the range of 3 to 10.[2]

Sulforhodamine B is often used as a membrane-impermeable polar tracer[3] or used for cell density determination via determination of cellular proteins (cytotoxicity assay).[4]

References[edit]

  1. ^ a b "Sulforhodamine B sodium salt (CAS 3520-42-1)". Santa Cruz Biotechnology. 
  2. ^ Coppeta, J.; Rogers, C. (1998). "Dual Emission Laser Induced Fluorescence for Direct Planar Scalar Behavior Measurements". Experiments in Fluids 25: 1–15. doi:10.1007/s003480050202. 
  3. ^ Viricel W; Mbarek A; Leblond J (2015). "Switchable Lipids: Conformational Change for Fast pH-Triggered Cytoplasmic Delivery". Angewandte Chemie International Edition 54: 12743–12747. doi:10.1002/anie.201504661. 
  4. ^ Vichai V; Kirtikara K (2006). "Sulforhodamine B colorimetric assay for cytotoxicity screening". Nature Protocols 1: 1112–1116. doi:10.1038/nprot.2006.179.