Susumu Ohno was born of Japanese parents in Seoul, Korea, on February 1, 1928. The second of five children, he was the son of the minister of education of the Japanese Protectorate of Korea. The family returned to Japan after the war in 1945. He later became a citizen of the United States Susumu Ohno married musician Midori Aoyama in 1951. They had two sons and one daughter.
His passion for science derived from his lifelong love of horses. He earned a Ph.D. in veterinary science at Tokyo University of Agriculture and Technology in 1949, and later a Ph.D. and D.Sc. from Hokkaido University. He went to the United States in 1951, as a visiting scholar to UCLA, and in 1952 joined the new research department at City of Hope Medical Center, where he remained in active research until 1996.
Ohno postulated that gene duplication plays a major role in evolution in his classic book Evolution by Gene Duplication (1970). While subsequent research has overwhelmingly confirmed the key role of gene duplication in molecular evolution, research to evaluate Ohno's model for the preservation of duplicate genes (now termed neofunctionalization) is ongoing and very active. He also discovered in 1956 that the Barr body of mammalian female nuclei was in fact a condensed X chromosome. In Evolution by Gene Duplication, he also suggested that vertebrate genome is the result of one or more entire genome duplications; variations of this idea have come to be known as the 2R hypothesis (also called "Ohno's hypothesis"). He indicated that mammalian X chromosomes are conserved among species; it has been referred to as Ohno's law. He also popularized the term junk DNA for segments of the DNA that have no known function.
In 1986, Ohno authored a paper published in Immunogenetics that explored the relationship between DNA genetic sequences and music. "The SARC oncogene, a malignant gene first discovered in chickens, causes cancer in humans as well. When Ohno translated the gene to music, it sounded very much like Chopin`s Funeral March". "An enzyme (phosphoglyceratekinase), which breaks down sugar (glucose) in the body revealed itself to Ohno as a lullaby. ``A violinist recorded the tune, and when kindergarten teachers in Tokyo play it, their youngsters yawn and willingly take their naps,``" said Ohno. The biologist, with no formal training in music, "decided to assign notes according to the molecular weights" and "put the heavier molecules in lower positions, and the lighter molecules higher". With DNA being composed of four subunits, he mapped each to two positions on the musics staff, forming an octave. He found that the more evolved an organism is, the more complicated is the music. His ultimate hope was "to find is some basic pattern that governs all life. . .everything." 
- Susumu Ohno (1970). Evolution by gene duplication. Springer-Verlag. ISBN 0-04-575015-7.
- Ohno S, Kaplan WD, Kinosita R (1959). "Formation of the sex chromatin by a single X-chromosome in liver cells of Rattus norvegicus". Experimental Cell Research. 18 (2): 415–418. doi:10.1016/0014-4827(59)90031-X. PMID 14428474.
- Ohno S (1967). Sex Chromosomes and Sex-Linked Genes. Berlin, New York: Springer-Verlag
- Wycoff, Mick (2008). "Art as Science Project: The Evolution of Darwin by Esther Solondz". Evolution: Education and Outreach. 1 (2): 210–222. doi:10.1007/s12052-008-0043-x.
- Ohno, S (1972). "So much "junk" DNA in our genome". Brookhaven Symposia in Biology. 23: 366–70. PMID 5065367.
- Gorner, Peter (April 16, 1986). "Genesis And Genes, Composing The Newest Testament Of Life". Chicago Tribune. DUARTE, CALIF. Retrieved Aug 1, 2015.