TGF beta signaling pathway
The transforming growth factor beta (TGFB) signaling pathway is involved in many cellular processes in both the adult organism and the developing embryo including cell growth, cell differentiation, apoptosis, cellular homeostasis and other cellular functions. In spite of the wide range of cellular processes that the TGFβ signaling pathway regulates, the process is relatively simple. TGFβ superfamily ligands bind to a type II receptor, which recruits and phosphorylates a type I receptor. The type I receptor then phosphorylates receptor-regulated SMADs (R-SMADs) which can now bind the coSMAD SMAD4. R-SMAD/coSMAD complexes accumulate in the nucleus where they act as transcription factors and participate in the regulation of target gene expression.
The TGF beta superfamily of ligands include: Bone morphogenetic proteins (BMPs), Growth and differentiation factors (GDFs), Anti-müllerian hormone (AMH), Activin, Nodal and TGFβ's. Signaling begins with the binding of a TGF beta superfamily ligand to a TGF beta type II receptor. The type II receptor is a serine/threonine receptor kinase, which catalyzes the phosphorylation of the Type I receptor. Each class of ligand binds to a specific type II receptor. In mammals there are seven known type I receptors and five type II receptors.
There are three activins: Activin A, Activin B and Activin AB. Activins are involved in embryogenesis and osteogenesis. They also regulate many hormones including pituitary, gonadal and hypothalamic hormones as well as insulin. They are also nerve cell survival factors.
The BMPs bind to the bone morphogenetic protein receptor type-2 (BMPR2). They are involved in a multitude of cellular functions including osteogenesis, cell differentiation, anterior/posterior axis specification, growth, and homeostasis.
The TGF beta family include: TGFβ1, TGFβ2, TGFβ3. Like the BMPs, TGF betas are involved in embryogenesis and cell differentiation, but they are also involved in apoptosis, as well as other functions. They bind to TGF-beta receptor type-2 (TGFBR2).
When the receptor-ligand binding occurs via local action, this is classified as paracrine signalling.
Receptor recruitment and phosphorylation
The TGF beta ligand binds to a type II receptor dimer, which recruits a type I receptor dimer forming a hetero-tetrameric complex with the ligand. These receptors are serine/threonine kinase receptors. They have a cysteine rich extracellular domain, a transmembrane domain and a cytoplasmic serine/threonine rich domain. The GS domain of the type I receptor consists of a series of about thirty serine-glycine repeats. The binding of a TGF beta family ligand causes the rotation of the receptors so that their cytoplasmic kinase domains are arranged in a catalytically favorable orientation. The Type II receptor phosphorylates serine residues of the Type I receptor, which activates the protein.
There are five receptor regulated SMADs: SMAD1, SMAD2, SMAD3, SMAD5, and SMAD9 (sometimes referred to as SMAD8). There are essentially two intracellular pathways involving these R-SMADs. TGF beta's, Activins, Nodals and some GDFs are mediated by SMAD2 and SMAD3, while BMPs, AMH and a few GDFs are mediated by SMAD1, SMAD5 and SMAD9. The binding of the R-SMAD to the type I receptor is mediated by a zinc double finger FYVE domain containing protein. Two such proteins that mediate the TGF beta pathway include SARA (The SMAD anchor for receptor activation) and HGS (Hepatocyte growth factor-regulated tyrosine kinase substrate).
SARA is present in an early endosome which, by clathrin-mediated endocytosis, internalizes the receptor complex. SARA recruits an R-SMAD. SARA permits the binding of the R-SMAD to the L45 region of the Type I receptor. SARA orients the R-SMAD such that serine residue on its C-terminus faces the catalytic region of the Type I receptor. The Type I receptor phosphorylates the serine residue of the R-SMAD. Phosphorylation induces a conformational change in the MH2 domain of the R-SMAD and its subsequent dissociation from the receptor complex and SARA.
The phosphorylated RSMAD has a high affinity for a coSMAD (e.g. SMAD4) and forms a complex with one. The phosphate group does not act as a docking site for coSMAD, rather the phosphorylation opens up an amino acid stretch allowing interaction.
The phosphorylated RSMAD/coSMAD complex enters the nucleus where it binds transcription promoters/cofactors and causes the transcription of DNA.
Activin causes the transcription of mRNAs involved in gonadal growth, embryo differentiation and placenta formation.
The TGF beta signaling pathway is involved in a wide range of cellular process and subsequently is very heavily regulated. There are a variety of mechanisms where the pathway is modulated either positively or negatively: There are agonists for ligands and R-SMADs; there are decoy receptors; and R-SMADs and receptors are ubiquitinated.
Both chordin and noggin are antagonists of BMPs. They bind BMPs preventing the binding of the ligand to the receptor. It has been demonstrated that Chordin and Noggin dorsalize mesoderm. They are both found in the dorsal lip of Xenopus and convert otherwise epidermis specified tissue into neural tissue (see neurulation). Noggin plays a key role in cartilage and bone patterning. Mice Noggin-/- have excess cartilage and lacked joint formation.
Members of the DAN family of proteins also antagonize TGF beta family members. They include Cerberus, DAN, and Gremlin. These proteins contain nine conserved cysteines which can form disulfide bridges. It is believed that DAN antagonizes GDF5, GDF6 and GDF7.
Follistatin inhibits Activin, which it binds. It directly affects follicle-stimulating hormone (FSH) secretion. Follistatin also is implicated in prostate cancers where mutations in its gene may preventing it from acting on activin which has anti-proliferative properties.
Lefty is a regulator of TGFβ and is involved in the axis patterning during embryogenesis. It is also a member of the TGF superfamily of proteins. It is asymmetrically expressed in the left side of murine embryos and subsequently plays a role in left-right specification. Lefty acts by preventing the phosphorylation of R-SMADs. It does so through a constitutively active TGFβ type I receptor and through a process downstream of its activation.
Drug-based antagonists have also been identified, such as SB431542, which selectively inhibits ALK4, ALK5, and ALK7.
The transforming growth factor receptor 3 (TGFBR3) is the most abundant of the TGF-β receptors yet, it has no known signaling domain. It however may serve to enhance the binding of TGF beta ligands to TGF beta type II receptors by binding TGFβ and presenting it to TGFBR2. One of the downstream targets of TGF β signaling, GIPC, binds to its PDZ domain, which prevents its proteosomal degradation, which subsequently increases TGFβ activity. It may also serve as an inhibin coreceptor to ActivinRII.
BMP and activin membrane bound inhibitor (BAMBI), has a similar extracellular domain as type I receptors. It lacks an intracellular serine/threonine protein kinase domain and hence is a pseudoreceptor. It binds to the type I receptor preventing it from being activated. It serves as a negative regulator of TGF beta signaling and may limit tgf-beta expression during embryogeneis. It requires BMP signaling for its expression
FKBP12 binds the GS region of the type I receptor preventing phosphorylation of the receptor by the type II receptors. It is believed that FKBP12 and its homologs help to prevent type I receptor activation in the absence of a ligands, since ligand binding causes its dissociation.
Role of inhibitory SMADs
There are two other SMADs which complete the SMAD family, the inhibitory SMADs (I-SMADS), SMAD6 and SMAD7. They play a key role in the regulation of TGF beta signaling and are involved in negative feedback. Like other SMADs they have an MH1 and an MH2 domain. SMAD7 competes with other R-SMADs with the Type I receptor and prevents their phosphorylation. It resides in the nucleus and upon TGF beta receptor activation translocates to the cytoplasm where it binds the type I receptor. SMAD6 binds SMAD4 preventing the binding of other R-SMADs with the coSMAD. The levels of I-SMAD increase with TGF beta signaling suggesting that they are downstream targets of TGF-beta signaling.
The E3 ubiquitin-protein ligases SMURF1 and SMURF2 regulate the levels of SMADs. They accept ubiquitin from an E2 conjugating enzyme where they transfer ubiquitin to the RSMADs which causes their ubiquitination and subsequent proteosomal degradation. SMURF1 binds to SMAD1 and SMAD5 while SMURF2 binds SMAD1, SMAD2, SMAD3, SMAD6 and SMAD7. It[clarification needed] enhances the inhibitory action of SMAD7 while reducing the transcriptional activities of SMAD2.
TGF-β ligands of H.sapiens highlighted in grey, of D.melanogaster ligands in pink, of C.elegans in yellow.
|TGF-β superfamily ligand||Ligand inhibitors||Type II Receptor||Type I receptor||R-SMADs||coSMAD||I-SMADs|
|Activin A||Follistatin||ACVR2A||ACVR1B (ALK4)||SMAD2, SMAD3||SMAD4||SMAD7|
|GDF1||ACVR2A||ACVR1B (ALK4)||SMAD2, SMAD3||SMAD4||SMAD7|
|GDF11||ACVR2B||ACVR1B (ALK4), TGFβRI (ALK5)||SMAD2, SMAD3||SMAD4||SMAD7|
|BMP2-8||Noggin, Chordin, DAN||BMPR2||BMPR1A (ALK3), BMPR1B (ALK6)||SMAD1 SMAD5, SMAD8||SMAD4||SMAD6, SMAD7|
|Nodal||Lefty||ACVR2B||ACVR1B (ALK4), ACVR1C (ALK7)||SMAD2, SMAD3||SMAD4||SMAD7|
|TGFβs||LTBP1, THBS1, Decorin||TGFβRII||TGFβRI (ALK5)||SMAD2, SMAD3||SMAD4||SMAD7|
|Gbb||Wit, Punt||Tkv, Sax||Mad||Medea|
|Dbl-1||Daf-4||Sma-6||Sma-2, Sma-3, Sma-4||Sma-4|
- Kyoto Encyclopedia of Genes and Genomes -TGF beta signaling pathway map
- Netpath - A curated resource of signal transduction pathways in humans
- "Prosite Documentation PDOC00223". Archived from the original on 2011-05-25. Retrieved 2006-07-01.
- Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P (2002). Molecular Biology of the Cell. New York, NY: Garland Science. ISBN 978-0-8153-3218-3.
- Munir S, Xu G, Wu Y, Yang B, Lala PK, Peng C (July 2004). "Nodal and ALK7 inhibit proliferation and induce apoptosis in human trophoblast cells". The Journal of Biological Chemistry. 279 (30): 31277–86. doi:10.1074/jbc.M400641200. PMID 15150278.
- Wrana JL, Attisano L, Cárcamo J, Zentella A, Doody J, Laiho M, et al. (December 1992). "TGF beta signals through a heteromeric protein kinase receptor complex". Cell. 71 (6): 1003–14. doi:10.1016/0092-8674(92)90395-S. PMID 1333888.
- "Pfam entry TGF_beta_GS". Retrieved 2006-07-01.
- Runyan CE, Schnaper HW, Poncelet AC (March 2005). "The role of internalization in transforming growth factor beta1-induced Smad2 association with Smad anchor for receptor activation (SARA) and Smad2-dependent signaling in human mesangial cells". The Journal of Biological Chemistry. 280 (9): 8300–8. doi:10.1074/jbc.M407939200. PMID 15613484.
- Moustakas A (September 2002). "Smad signalling network". Journal of Cell Science. 115 (Pt 17): 3355–6. PMID 12154066.
- Souchelnytskyi S, Rönnstrand L, Heldin CH, ten Dijke P (2001). "Phosphorylation of Smad signaling proteins by receptor serine/threonine kinases". Protein Kinase Protocols. Methods in Molecular Biology. 124. pp. 107–20. doi:10.1385/1-59259-059-4:107. ISBN 1-59259-059-4. PMID 11100470.
- Massagué J, Chen YG (March 2000). "Controlling TGF-beta signaling". Genes & Development. 14 (6): 627–44. PMID 10733523.
- Ulloa L, Tabibzadeh S (June 2001). "Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor". The Journal of Biological Chemistry. 276 (24): 21397–404. doi:10.1074/jbc.M010783200. PMID 11278746.
- Laping NJ, Grygielko E, Mathur A, Butter S, Bomberger J, Tweed C, et al. (July 2002). "Inhibition of transforming growth factor (TGF)-beta1-induced extracellular matrix with a novel inhibitor of the TGF-beta type I receptor kinase activity: SB-431542". Molecular Pharmacology. 62 (1): 58–64. doi:10.1124/mol.62.1.58. PMID 12065755.
- Blobe GC, Liu X, Fang SJ, How T, Lodish HF (October 2001). "A novel mechanism for regulating transforming growth factor beta (TGF-beta) signaling. Functional modulation of type III TGF-beta receptor expression through interaction with the PDZ domain protein, GIPC". The Journal of Biological Chemistry. 276 (43): 39608–17. doi:10.1074/jbc.M106831200. PMID 11546783.
- Online Mendelian Inheritance in Man (OMIM): TRANSFORMING GROWTH FACTOR-BETA RECEPTOR, TYPE III; TGFBR3 - 600742
- Itoh F, Asao H, Sugamura K, Heldin CH, ten Dijke P, Itoh S (August 2001). "Promoting bone morphogenetic protein signaling through negative regulation of inhibitory Smads". The EMBO Journal. 20 (15): 4132–42. doi:10.1093/emboj/20.15.4132. PMC 149146. PMID 11483516.