TPCN1

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TPCN1
Identifiers
Aliases TPCN1, TPC1, two pore segment channel 1
External IDs MGI: 2182472 HomoloGene: 9905 GeneCards: TPCN1
Targeted by Drug
phosphatidyl (3,5) inositol biphosphate, verapamil[1]
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001143819
NM_001301214
NM_017901

NM_145853

RefSeq (protein)

NP_001137291.1
NP_001288143.1
NP_060371.2

NP_665852.1

Location (UCSC) Chr 12: 113.22 – 113.3 Mb Chr 5: 120.53 – 120.59 Mb
PubMed search [2] [3]
Wikidata
View/Edit Human View/Edit Mouse

Two pore segment channel 1 (TPC1) is a human protein encoded by the TPCN1 gene.[4] The protein encoded by this gene is an ion channel. In contrast to other calcium and sodium channels which have four homologous domains, each containing 6 transmembrane segments (S1 to S6), TPCN1 only contains two domains (each containing segments S1 to S6).[5][6][7]

Structure[edit]

The structure of a TPC1 ortholog from Arabidopsis thaliana has been solved by two laboratories.[8][9] The structures were solved using X-ray crystallography and contained the fold of a voltage-gated ion channel and EF hands. Only a single voltage sensor domain appears to responsible for voltage sensing.

Filoviral Infections[edit]

Genetic knockout and pharmacological inhibition experiments demonstrate that Two-pore Channels, TPC1 and TPC2, are required for infection by Filoviruses Ebola and Marburg in mice.[10]

See also[edit]

References[edit]

  1. ^ "Drugs that physically interact with Two pore calcium channel protein 1 view/edit references on wikidata". 
  2. ^ "Human PubMed Reference:". 
  3. ^ "Mouse PubMed Reference:". 
  4. ^ "Entrez Gene: two pore segment channel 1". 
  5. ^ Hirosawa M, Nagase T, Ishikawa K, Kikuno R, Nomura N, Ohara O (October 1999). "Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain". DNA Research. 6 (5): 329–36. doi:10.1093/dnares/6.5.329. PMID 10574461. 
  6. ^ Ishibashi K, Suzuki M, Imai M (April 2000). "Molecular cloning of a novel form (two-repeat) protein related to voltage-gated sodium and calcium channels". Biochemical and Biophysical Research Communications. 270 (2): 370–6. doi:10.1006/bbrc.2000.2435. PMID 10753632. 
  7. ^ Clapham DE, Garbers DL (December 2005). "International Union of Pharmacology. L. Nomenclature and structure-function relationships of CatSper and two-pore channels". Pharmacological Reviews. 57 (4): 451–4. doi:10.1124/pr.57.4.7. PMID 16382101. 
  8. ^ Guo J, Zeng W, Chen Q, Lee C, Chen L, Yang Y, Cang C, Ren D, Jiang Y (March 2016). "Structure of the voltage-gated two-pore channel TPC1 from Arabidopsis thaliana". Nature. 531 (7593): 196–201. doi:10.1038/nature16446. PMC 4841471Freely accessible. PMID 26689363. 
  9. ^ Kintzer AF, Stroud RM (March 2016). "Structure, inhibition and regulation of two-pore channel TPC1 from Arabidopsis thaliana". Nature. 531 (7593): 258–62. doi:10.1038/nature17194. PMID 26961658. 
  10. ^ Sakurai Y, Kolokoltsov AA, Chen CC, Tidwell MW, Bauta WE, Klugbauer N, Grimm C, Wahl-Schott C, Biel M, Davey RA (February 2015). "Ebola virus. Two-pore channels control Ebola virus host cell entry and are drug targets for disease treatment". Science. 347 (6225): 995–8. doi:10.1126/science.1258758. PMC 4550587Freely accessible. PMID 25722412. 

Further reading[edit]

  • Ishibashi K, Suzuki M, Imai M (April 2000). "Molecular cloning of a novel form (two-repeat) protein related to voltage-gated sodium and calcium channels". Biochemical and Biophysical Research Communications. 270 (2): 370–6. doi:10.1006/bbrc.2000.2435. PMID 10753632. 
  • Hirosawa M, Nagase T, Ishikawa K, Kikuno R, Nomura N, Ohara O (October 1999). "Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain". DNA Research. 6 (5): 329–36. doi:10.1093/dnares/6.5.329. PMID 10574461. 
  • Clapham DE, Garbers DL (December 2005). "International Union of Pharmacology. L. Nomenclature and structure-function relationships of CatSper and two-pore channels". Pharmacological Reviews. 57 (4): 451–4. doi:10.1124/pr.57.4.7. PMID 16382101. 
  • Calcraft PJ, Ruas M, Pan Z, Cheng X, Arredouani A, Hao X, Tang J, Rietdorf K, Teboul L, Chuang KT, Lin P, Xiao R, Wang C, Zhu Y, Lin Y, Wyatt CN, Parrington J, Ma J, Evans AM, Galione A, Zhu MX (May 2009). "NAADP mobilizes calcium from acidic organelles through two-pore channels". Nature. 459 (7246): 596–600. doi:10.1038/nature08030. PMC 2761823Freely accessible. PMID 19387438. 
  • Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (June 2002). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Research. 9 (3): 99–106. doi:10.1093/dnares/9.3.99. PMID 12168954. 

External links[edit]