TRAIL

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TNFSF10
Protein TNFSF10 PDB 1d0g.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases TNFSF10, APO2L, Apo-2L, CD253, TL2, TRAIL, TNLG6A, tumor necrosis factor superfamily member 10
External IDs OMIM: 603598 MGI: 107414 HomoloGene: 2824 GeneCards: 8743
RNA expression pattern
PBB GE TNFSF10 202687 s at tn.png

PBB GE TNFSF10 202688 at tn.png

PBB GE TNFSF10 214329 x at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001190942
NM_001190943
NM_003810

NM_009425

RefSeq (protein)

NP_001177871.1
NP_003801.1

NP_033451.1

Location (UCSC) Chr 3: 172.51 – 172.52 Mb Chr 3: 27.32 – 27.34 Mb
PubMed search [1] [2]
Wikidata
View/Edit Human View/Edit Mouse

In the field of cell biology, TNF-related apoptosis-inducing ligand (TRAIL), is a protein functioning as a ligand that induces the process of cell death called apoptosis.[1][2]

TRAIL is a cytokine that is produced and secreted by most normal tissue cells. It causes apoptosis primarily in tumor cells,[3] by binding to certain death receptors. TRAIL and its receptors have been used as the targets of several anti-cancer therapeutics since the mid-1990s, such as Mapatumumab. However, as of 2013, these have not shown significant survival benefit.[4]

TRAIL has also been designated CD253 (cluster of differentiation 253) and TNFSF10 (tumor necrosis factor (ligand) superfamily, member 10).[3]

Gene[edit]

In humans, the gene that encodes TRAIL is located at chromosome 3q26, which is not close to other TNF family members.[1] The genomic structure of the TRAIL gene spans approximately 20 kb and is composed of five exonic segments 222, 138, 42, 106, and 1245 nucleotides and four introns of approximately 8.2, 3.2, 2.3 and 2.3 kb.

The TRAIL gene lacks TATA and CAAT boxes and the promotor region contains putative response elements for transcription factors GATA, AP-1, C/EBP, SP-1, OCT-1, AP3, PEA3, CF-1, and ISRE.[citation needed]

The TRAIL gene as a drug target[edit]

TIC10 (which causes expression of TRAIL) was investigated in mice with various tumour types.[4]

Small molecule ONC201 causes expression of TRAIL which kills some cancer cells.[5]

Structure[edit]

TRAIL shows homology to other members of the tumor necrosis factor superfamily. It is composed of 281 amino acids and has characteristics of a type II transmembrane protein (i.e. no leader sequence and an internal transmembrane domain). The N-terminal cytoplasmic domain is not conserved across family members, however, the C-terminal extracellular domain is conserved and can be proteolytically cleaved from the cell surface. TRAIL forms a homotrimer that binds three receptor molecules.

Function[edit]

TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases.[6] TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2). DcR1 functions as a TRAIL-neutralizing decoy-receptor. The cytoplasmic domain of DcR2 is functional and activates NFkappaB. In cells expressing DcR2, TRAIL binding therefore activates NFkappaB, leading to transcription of genes known to antagonize the death signaling pathway and/or to promote inflammation.

The TRAIL receptors as a drug target[edit]

In clinical trials only a small proportion of patients responded to various drugs that targeted TRAIL death receptors.[7]

Interactions[edit]

TRAIL has been shown to interact with TNFRSF10B.[8][9][10]

See also[edit]

References[edit]

  1. ^ a b Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK, Sutherland GR, Smith TD, Rauch C, Smith CA (December 1995). "Identification and characterization of a new member of the TNF family that induces apoptosis". Immunity 3 (6): 673–82. doi:10.1016/1074-7613(95)90057-8. PMID 8777713. 
  2. ^ Pitti RM, Marsters SA, Ruppert S, Donahue CJ, Moore A, Ashkenazi A (May 1996). "Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family". J. Biol. Chem. 271 (22): 12687–90. doi:10.1074/jbc.271.22.12687. PMID 8663110. 
  3. ^ a b "TNFSF10". NCBI Gene. 
  4. ^ a b Cormier Z (February 2013). "Small-molecule drug drives cancer cells to suicide". Nature 494. doi:10.1038/nature.2013.12385. 
  5. ^ ONC201: Stressing tumors to death. Feb 2016
  6. ^ Song JJ, Lee YJ (May 2008). "Differential cleavage of Mst1 by caspase-7/-3 is responsible for TRAIL-induced activation of the MAPK superfamily". Cell. Signal. 20 (5): 892–906. doi:10.1016/j.cellsig.2008.01.001. PMC 2483832. PMID 18276109. 
  7. ^ "On the TRAIL to successful cancer therapy? Predicting and counteracting resistance against TRAIL-based therapeutics.". Oncogene 32, 1341-1350. 14 March 2013. doi:10.1038/onc.2012.164. 
  8. ^ Kaptein A, Jansen M, Dilaver G, Kitson J, Dash L, Wang E, Owen MJ, Bodmer JL, Tschopp J, Farrow SN (November 2000). "Studies on the interaction between TWEAK and the death receptor WSL-1/TRAMP (DR3)". FEBS Lett. 485 (2-3): 135–41. doi:10.1016/S0014-5793(00)02219-5. PMID 11094155. 
  9. ^ Walczak H, Degli-Esposti MA, Johnson RS, Smolak PJ, Waugh JY, Boiani N, Timour MS, Gerhart MJ, Schooley KA, Smith CA, Goodwin RG, Rauch CT (September 1997). "TRAIL-R2: a novel apoptosis-mediating receptor for TRAIL". EMBO J. 16 (17): 5386–97. doi:10.1093/emboj/16.17.5386. PMC 1170170. PMID 9311998. 
  10. ^ Hymowitz SG, Christinger HW, Fuh G, Ultsch M, O'Connell M, Kelley RF, Ashkenazi A, de Vos AM (October 1999). "Triggering cell death: the crystal structure of Apo2L/TRAIL in a complex with death receptor 5". Mol. Cell 4 (4): 563–71. doi:10.1016/S1097-2765(00)80207-5. PMID 10549288. 

Further reading[edit]

  • Almasan A, Ashkenazi A (2004). "Apo2L/TRAIL: apoptosis signaling, biology, and potential for cancer therapy". Cytokine Growth Factor Rev. 14 (3–4): 337–48. doi:10.1016/S1359-6101(03)00029-7. PMID 12787570. 
  • Cha SS, Song YL, Oh BH (2004). "Specificity of molecular recognition learned from the crystal structures of TRAIL and the TRAIL:sDR5 complex". Vitam. Horm. Vitamins & Hormones 67: 1–17. doi:10.1016/S0083-6729(04)67001-4. ISBN 978-0-12-709867-8. PMID 15110168. 
  • Song C, Jin B (2005). "TRAIL (CD253), a new member of the TNF superfamily". J. Biol. Regul. Homeost. Agents 19 (1–2): 73–7. PMID 16178278. 
  • Bucur O, Ray S, Bucur MC, Almasan A (2006). "APO2 ligand/tumor necrosis factor-related apoptosis-inducing ligand in prostate cancer therapy". Front. Biosci. 11: 1549–68. doi:10.2741/1903. PMID 16368536. 

External links[edit]