|RNA type||gene, tRNA|
A transfer RNA (abbreviated tRNA and formerly referred to as sRNA, for soluble RNA) is an adaptor molecule composed of RNA, typically 76 to 90 nucleotides in length, that serves as the physical link between the mRNA and the amino acid sequence of proteins. It does this by carrying an amino acid to the protein synthetic machinery of a cell (ribosome) as directed by a three-nucleotide sequence (codon) in a messenger RNA (mRNA). As such, tRNAs are a necessary component of translation, the biological synthesis of new proteins in accordance with the genetic code.
While the specific nucleotide sequence of an mRNA specifies which amino acids are incorporated into the protein product of the gene from which the mRNA is transcribed, the role of tRNA is to specify which sequence from the genetic code corresponds to which amino acid. The mRNA encodes a protein as a series of contiguous codons, each of which is recognized by a particular tRNA. One end of the tRNA matches the genetic code in a three-nucleotide sequence called the anticodon. The anticodon forms three base pairs with a codon in mRNA during protein biosynthesis. On the other end of the tRNA is a covalent attachment to the amino acid that corresponds to the anticodon sequence. Each type of tRNA molecule can be attached to only one type of amino acid, so each organism has many types of tRNA. Because the genetic code contains multiple codons that specify the same amino acid, there are several tRNA molecules bearing different anticodons which carry the same amino acid.
The covalent attachment to the tRNA 3’ end is catalyzed by enzymes called aminoacyl tRNA synthetases. During protein synthesis, tRNAs with attached amino acids are delivered to the ribosome by proteins called elongation factors, which aid in association of the tRNA with the ribosome, synthesis of the new polypeptide and translocation (movement) of the ribosome along the mRNA. If the tRNA's anticodon matches the mRNA, another tRNA already bound to the ribosome transfers the growing polypeptide chain from its 3’ end to the amino acid attached to the 3’ end of the newly delivered tRNA, a reaction catalyzed by the ribosome. A large number of the individual nucleotides in a tRNA molecule may be chemically modified, often by methylation or deamidation. These unusual bases sometimes affect the tRNA's interaction with ribosomes and sometimes occur in the anticodon to alter base-pairing properties.:29.1.2
The structure of tRNA can be decomposed into its primary structure, its secondary structure (usually visualized as the cloverleaf structure), and its tertiary structure (all tRNAs have a similar L-shaped 3D structure that allows them to fit into the P and A sites of the ribosome). The cloverleaf structure becomes the 3D L-shaped structure through coaxial stacking of the helices, which is a common RNA tertiary structure motif.
The tRNA structure consists of the following:
- A 5'-terminal phosphate group.
- The acceptor stem is a 7- to 9-base pair (bp) stem made by the base pairing of the 5'-terminal nucleotide with the 3'-terminal nucleotide (which contains the CCA 3'-terminal group used to attach the amino acid). The acceptor stem may contain non-Watson-Crick base pairs.
- The CCA tail is a cytosine-cytosine-adenine sequence at the 3' end of the tRNA molecule. The amino acid loaded onto the tRNA by aminoacyl tRNA synthetases, to form aminoacyl-tRNA, is covalently bonded to the 3'-hydroxyl group on the CCA tail. This sequence is important for the recognition of tRNA by enzymes and critical in translation. In prokaryotes, the CCA sequence is transcribed in some tRNA sequences. In most prokaryotic tRNAs and eukaryotic tRNAs, the CCA sequence is added during processing and therefore does not appear in the tRNA gene.
- The D arm is a 4- to 6-bp stem ending in a loop that often contains dihydrouridine.
- The anticodon arm is a 5-bp stem whose loop contains the anticodon. The tRNA 5'-to-3' primary structure contains the anticodon but in reverse order, since 3'-to-5' directionality is required to read the mRNA from 5'-to-3'.
- The T arm is a 4- to 5- bp stem containing the sequence TΨC where Ψ is pseudouridine, a modified uridine.
- Bases that have been modified, especially by methylation (e.g. tRNA (guanine-N7-)-methyltransferase), occur in several positions throughout the tRNA. The first anticodon base, or wobble-position, is sometimes modified to inosine (derived from adenine), pseudouridine or lysidine (derived from cytosine).
An anticodon is a unit made up of three nucleotides that correspond to the three bases of the codon on the mRNA. Each tRNA contains a distinct anticodon triplet sequence that can base-pair to one or more codons for an amino acid. Some anticodons can pair with more than one codon due to a phenomenon known as wobble base pairing. Frequently, the first nucleotide of the anticodon is one not found on mRNA: inosine, which can hydrogen bond to more than one base in the corresponding codon position.:29.3.9 In the genetic code, it is common for a single amino acid to be specified by all four third-position possibilities, or at least by both pyrimidines and purines; for example, the amino acid glycine is coded for by the codon sequences GGU, GGC, GGA, and GGG. Other modified nucleotides may also appear at the first anticodon position - sometimes known as the "wobble position" - resulting in subtle changes to the genetic code, as for example in mitochondria.
To provide a one-to-one correspondence between tRNA molecules and codons that specify amino acids, 61 types of tRNA molecules would be required per cell. However, many cells contain fewer than 61 types of tRNAs because the wobble base is capable of binding to several, though not necessarily all, of the codons that specify a particular amino acid. A minimum of 31 tRNA are required to translate, unambiguously, all 61 sense codons of the standard genetic code.
Each tRNA is aminoacylated (or charged) with a specific amino acid by an aminoacyl tRNA synthetase. There is normally a single aminoacyl tRNA synthetase for each amino acid, despite the fact that there can be more than one tRNA, and more than one anticodon, for an amino acid. Recognition of the appropriate tRNA by the synthetases is not mediated solely by the anticodon, and the acceptor stem often plays a prominent role.
Certain organisms can have one or more aminoacyl tRNA synthetases missing. This leads to charging of the tRNA by a chemically related amino acid. An enzyme or enzymes modify the charged amino acid to the final one. For example, Helicobacter pylori has glutaminyl tRNA synthetase missing. Thus, glutamate tRNA synthetase charges tRNA-glutamine(tRNA-Gln) with glutamate. An amidotransferase then converts the acid side chain of the glutamate to the amide, forming the correctly charged gln-tRNA-Gln.
Binding to ribosome
The ribosome has three binding sites for tRNA molecules that span the space between the two ribosomal subunits: the A (aminoacyl), P (peptidyl), and E (exit) sites. In addition, the ribosome has two other sites for tRNA binding that are used during mRNA decoding or during the initiation of protein synthesis. These are the T site (named elongation factor Tu) and I site (initiation). By convention, the tRNA binding sites are denoted with the site on the small ribosomal subunit listed first and the site on the large ribosomal subunit listed second. For example, the A site is often written A/A, the P site, P/P, and the E site, E/E. The binding proteins like L27, L2, L14, L15, L16 at the A- and P- sites have been determined by affinity labeling by A.P. Czernilofsky et al. (Proc. Natl. Acad. Sci, USA, pp 230–234, 1974).
Once translation initiation is complete, the first aminoacyl tRNA is located in the P/P site, ready for the elongation cycle described below. During translation elongation, tRNA first binds to the ribosome as part of a complex with elongation factor Tu (EF-Tu) or its eukaryotic (eEF-1) or archaeal counterpart. This initial tRNA binding site is called the A/T site. In the A/T site, the A-site half resides in the small ribosomal subunit where the mRNA decoding site is located. The mRNA decoding site is where the mRNA codon is read out during translation. The T-site half resides mainly on the large ribosomal subunit where EF-Tu or eEF-1 interacts with the ribosome. Once mRNA decoding is complete, the aminoacyl-tRNA is bound in the A/A site and is ready for the next peptide bond to be formed to its attached amino acid. The peptidyl-tRNA, which transfers the growing polypeptide to the aminoacyl-tRNA bound in the A/A site, is bound in the P/P site. Once the peptide bond is formed, the tRNA in the P/P site is deacylated, or has a free 3’ end, and the tRNA in the A/A site carries the growing polypeptide chain. To allow for the next elongation cycle, the tRNAs then move through hybrid A/P and P/E binding sites, before completing the cycle and residing in the P/P and E/E sites. Once the A/A and P/P tRNAs have moved to the P/P and E/E sites, the mRNA has also moved over by one codon and the A/T site is vacant, ready for the next round of mRNA decoding. The tRNA bound in the E/E site then leaves the ribosome.
The P/I site is actually the first to bind to aminoacyl tRNA, which is delivered by an initiation factor called IF2 in bacteria. However, the existence of the P/I site in eukaryotic or archaeal ribosomes has not yet been confirmed. The P-site protein L27 has been determined by affinity labeling by E. Collatz and A.P. Czernilofsky (FEBS Lett., Vol. 63, pp 283–286, 1976).
Organisms vary in the number of tRNA genes in their genome. The nematode worm C. elegans, a commonly used model organism in genetics studies, has 29,647  genes in its nuclear genome, of which 620 code for tRNA. The budding yeast Saccharomyces cerevisiae has 275 tRNA genes in its genome.
In the human genome, which, according to January 2013 estimates, has about 20,848 protein coding genes  in total, there are 497 nuclear genes encoding cytoplasmic tRNA molecules, and 324 tRNA-derived pseudogenes—tRNA genes thought to be no longer functional (although pseudo tRNAs have been shown to be involved in antibiotic resistance in bacteria ). Regions in nuclear chromosomes, very similar in sequence to mitochondrial tRNA genes, have also been identified (tRNA-lookalikes). These tRNA-lookalikes are also considered part of the nuclear mitochondrial DNA (genes transferred from the mitochondria to the nucleus).
Cytoplasmic tRNA genes can be grouped into 49 families according to their anticodon features. These genes are found on all chromosomes, except 22 and Y chromosome. High clustering on 6p is observed (140 tRNA genes), as well on 1 chromosome.
Genomic tRNA content is a differentiating feature of genomes among biological domains of life: Archaea present the simplest situation in terms of genomic tRNA content with a uniform number of gene copies, Bacteria have an intermediate situation and Eukarya present the most complex situation. Eukarya present not only more tRNA gene content than the other two kingdoms but also a high variation in gene copy number among different isoacceptors, and this complexity seem to be due to duplications of tRNA genes and changes in anticodon specificity.
Evolution of the tRNA gene copy number across different species has been linked to the appearance of specific tRNA modification enzymes (uridine methyltransferases in Bacteria, and adenosine deaminases in Eukarya), which increase the decoding capacity of a given tRNA. As an example, tRNAAla encodes four different tRNA isoacceptors (AGC, UGC, GGC and CGC). In Eukarya, AGC isoacceptors are extremely enriched in gene copy number in comparison to the rest of isoacceptors, and this has been correlated with its A-to-I modification of its wobble base. This same trend has been shown for most amino acids of eukaryal species. Indeed, the effect of these two tRNA modifications is also seen in codon usage bias. Highly expressed genes seem to be enriched in codons that are exclusively using codons that will be decoded by these modified tRNAs, which suggests a possible role of these codons—and consequently of these tRNA modifications—in translation efficiency. 
tRNA-derived fragments (or tRFs) are short molecules that emerge after cleavage of the mature tRNAs or the precursor transcript. Both cytoplasmic and mitochondrial tRNAs can produce fragments. There are at least four structural types of tRFs believed to originate from mature tRNAs, including the relatively long tRNA halves and short 5’-tRFs, 3’-tRFs and i-tRFs. The precursor tRNA can be cleaved to produce molecules from the 5’ leader or 3’ trail sequences. Cleavage enzymes include Angiogenin, Dicer, RNase Z and RNase P. Especially in the case of Angiogenin, the tRFs have a characteristically unusual cyclic phosphate at their 3’ end and a hydroxyl group at the 5’ end.
tRFs have multiple dependencies and roles. They exhibit significant changes between sexes, among races and disease status. Functionally, they can be loaded on Ago and act through RNAi pathways, participate in the formation of stress granules, displace mRNAs from RNA-binding proteins or inhibit translation. At the system or the organismal level, the four types of tRFs have a diverse spectrum of activities. Functionally, tRFs are associated with viral infection, cancer, cell proliferation  and also with epigenetic transgenerational regulation of metabolism.
Two online tools are available for those wishing to learn more about tRFs: the framework for the interactive exploration of mitochondrial and nuclear tRNA fragments (MINTbase) and the relational database of Transfer RNA related Fragments(tRFdb),. MINTbase also provides a naming scheme for the naming of tRFs called tRF-license plates that is genome independent.
In eukaryotic cells, tRNAs are transcribed by RNA polymerase III as pre-tRNAs in the nucleus. RNA polymerase III recognizes two highly conserved downstream promoter sequences: the 5' intragenic control region (5'-ICR, D-control region, or A box), and the 3'-ICR (T-control region or B box) inside tRNA genes. The first promoter begins at +8 of mature tRNAs and the second promoter is located 30-60 nucleotides downstream of the first promoter. The transcription terminates after a stretch of four or more thymidines.
Pre-tRNAs undergo extensive modifications inside the nucleus. Some pre-tRNAs contain introns that are spliced, or cut, to form the functional tRNA molecule; in bacteria these self-splice, whereas in eukaryotes and archaea they are removed by tRNA-splicing endonucleases. Eukaryotic pre-tRNA contains bulge-helix-bulge (BHB) structure motif that is important for recognition and precise splicing of tRNA intron by endonucleases. This motif position and structure are evolutionary conserved. However, some organisms, such as unicellular algae have a non-canonical position of BHB-motif as well as 5'- and 3'-ends of the spliced intron sequence. The 5' sequence is removed by RNase P, whereas the 3' end is removed by the tRNase Z enzyme. A notable exception is in the archaeon Nanoarchaeum equitans, which does not possess an RNase P enzyme and has a promoter placed such that transcription starts at the 5' end of the mature tRNA. The non-templated 3' CCA tail is added by a nucleotidyl transferase. Before tRNAs are exported into the cytoplasm by Los1/Xpo-t, tRNAs are aminoacylated. The order of the processing events is not conserved. For example, in yeast, the splicing is not carried out in the nucleus but at the cytoplasmic side of mitochondrial membranes.
The existence of tRNA was first hypothesized by Francis Crick, based on the assumption that there must exist an adapter molecule capable of mediating the translation of the RNA alphabet into the protein alphabet. Significant research on structure was conducted in the early 1960s by Alex Rich and Don Caspar, two researchers in Boston, the Jacques Fresco group in Princeton University and a United Kingdom group at King's College London. In 1965, Robert W. Holley of Cornell University reported the primary structure and suggested three secondary structures. tRNA was first crystallized in Madison, Wisconsin, by Robert M. Bock. The cloverleaf structure was ascertained by several other studies in the following years and was finally confirmed using X-ray crystallography studies in 1974. Two independent groups, Kim Sung-Hou working under Alexander Rich and a British group headed by Aaron Klug, published the same crystallography findings within a year.
- Plescia, O J; Palczuk, N C; Cora-Figueroa, E; Mukherjee, A; Braun, W (October 1965). "Production of antibodies to soluble RNA (sRNA)". Proc. Natl. Acad. Sci. USA. 54 (4): 1281–1285. doi:10.1073/pnas.54.4.1281. PMC . PMID 5219832.
- Sharp, Stephen J; Schaack, Jerome; Cooley, Lynn; Burke, Deborah J; Soll, Dieter (1985). "Structure and Transcription of Eukaryotic tRNA Genes". CRC Critical Reviews in Biochemistry. 19 (2): 107–144. doi:10.3109/10409238509082541. PMID 3905254. Retrieved 23 November 2014.
- Crick F (1968). "The origin of the genetic code". J Mol Biol. 38 (3): 367–379. doi:10.1016/0022-2836(68)90392-6. PMID 4887876.
- Stryer L, Berg JM, Tymoczko JL (2002). Biochemistry (5th ed.). San Francisco: W.H. Freeman. ISBN 0-7167-4955-6.
- Itoh, Yuzuru; Sekine, Shun-ichi Sekine; Suetsugu, Shiro; Yokoyama, Shigeyuki (6 May 2013). "Tertiary structure of bacterial selenocysteine tRNA". Nucleic Acids Research. 41 (13): 6729–6738. doi:10.1093/nar/gkt321. PMC . PMID 23649835. Retrieved 23 November 2014.
- Goodenbour, J. M.; Pan, T. (29 October 2006). "Diversity of tRNA genes in eukaryotes" (PDF). Nucleic Acids Research. 34 (21): 6137–6146. doi:10.1093/nar/gkl725. PMC . PMID 17088292. Retrieved 23 November 2014.
- Jahn, Martina; Rogers, M. John; Söll, Dieter (18 July 1991). "Anticodon and acceptor stem nucleotides in tRNAGln are major recognition elements for E. coli glutaminyl-tRNA synthetase". Nature. 352 (6332): 258–260. doi:10.1038/352258a0. PMID 1857423. Retrieved 23 November 2014.
- Ibba, Michael; Söll, Dieter (June 2000). "Aminoacyl-tRNA Synthesis". Annual Review of Biochemistry. 69 (1): 617–650. doi:10.1146/annurev.biochem.69.1.617. PMID 10966471. Retrieved 23 November 2014.
- Sprinzl, M., and Cramer, F. (1979) Prog. Nucleic Acids Res. Mol. Biol. 22, 1–16
- Green, R., and Noller, H. F. (1997) Annu. Rev. Biochem. 66, 679–716
- Aebi M, Kirchner G, Chen JY, et al. (September 1990). "Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae". J. Biol. Chem. 265 (27): 16216–16220. PMID 2204621.
- McCloskey, James A.; Nishimura, Susumu (November 1977). "Modified nucleosides in transfer RNA". Accounts of Chemical Research. 10 (11): 403–410. doi:10.1021/ar50119a004. Retrieved 23 November 2014.
- Felsenfeld G, Cantoni G; Cantoni (1964). "Use of thermal denaturation studies to investigate the base sequence of yeast serine sRNA". Proc Natl Acad Sci USA. 51 (5): 818–26. Bibcode:1964PNAS...51..818F. doi:10.1073/pnas.51.5.818. PMC . PMID 14172997.
- Suzuki, T; Suzuki, T (June 2014). "A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs.". Nucleic Acids Research. 42 (11): 7346–57. doi:10.1093/nar/gku390. PMID 24831542.
- Lodish H, Berk A, Matsudaira P, Kaiser CA, Krieger M, Scott MP, Zipursky SL, Darnell J. (2004). Molecular Biology of the Cell. WH Freeman: New York, NY. 5th ed.
- Schimmel P, Giege R, Moras D, Yokoyama S; Giege; Moras; Yokoyama (1993). "An operational RNA code for amino acids and possible relationship to genetic code". Proc. Natl. Acad. Sci. USA. 90 (19): 8763–876. Bibcode:1993PNAS...90.8763S. doi:10.1073/pnas.90.19.8763.
- Dunkle JA, Wang L, Feldman MB, Pulk A, Chen VB, Kapral GJ, Noeske J, Richardson JS, Blanchard SC, Cate JH; Wang; Feldman; Pulk; Chen; Kapral; Noeske; Richardson; Blanchard; Cate (2011). "Structures of the bacterial ribosome in classical and hybrid states of tRNA binding". Science. 332 (6032): 981–984. Bibcode:2011Sci...332..981D. doi:10.1126/science.1202692. PMC . PMID 21596992.
- Konevega, AL; Soboleva, NG; Makhno, VI; Semenkov, YP; Wintermeyer, W; Rodnina, MV; Katunin, VI (Jan 2004). "Purine bases at position 37 of tRNA stabilize codon-anticodon interaction in the ribosomal A site by stacking and Mg2+-dependent interactions". RNA. 10 (1): 90–101. doi:10.1261/rna.5142404. PMC . PMID 14681588.
- Agirrezabala X, Frank J; Frank (2009). "Elongation in translation as a dynamic interaction among the ribosome, tRNA, and elongation factors EF-G and EF-Tu". Q Rev Biophys. 42 (3): 159–200. doi:10.1017/S0033583509990060. PMC . PMID 20025795.
- Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J; Zavialov; Gursky; Ehrenberg; Frank (2005). "The cryo-EM structure of a translation initiation complex from Escherichia coli". Cell. 121 (5): 703–712. doi:10.1016/j.cell.2005.03.023. PMID 15935757.
- WormBase web site, http://www.wormbase.org, release WS187, date 25-Jan-2008.
- Spieth, J; Lawson, D (Jan 2006). "Overview of gene structure". WormBook: 1–10. doi:10.1895/wormbook.1.65.1. PMID 18023127.
- Hartwell LH, Hood L, Goldberg ML, Reynolds AE, Silver LM, Veres RC. (2004). Genetics: From Genes to Genomes 2nd ed. McGraw-Hill: New York, NY. p 264.
- Ensembl release 70 - Jan 2013 http://www.ensembl.org/Homo_sapiens/Info/StatsTable?db=core
- Lander E.; et al. (2001). "Initial sequencing and analysis of the human genome". Nature. 409 (6822): 860–921. doi:10.1038/35057062. PMID 11237011.
- Rogers Theresa E.; et al. (2012). "A Pseudo-tRNA Modulates Antibiotic Resistance in Bacillus cereus". PLoS ONE. 7 (7): e41248. doi:10.1371/journal.pone.0041248. PMID 22815980.
- Telonis Aristeidis G.; et al. (2014). "Nuclear and Mitochondrial tRNA-lookalikes in the Human Genome". Frontiers in Genetics. 5: 00344. doi:10.3389/fgene.2014.00344. PMC . PMID 25339973.
- Ramos A.; et al. (2011). "Nuclear Insertions of Mitochondrial Origin: Database Updating and Usefulness in Cancer Studies". Mitochondrion. 11 (6): 946–53. doi:10.1016/j.mito.2011.08.009. PMID 21907832.
- Ibid. p 529.
- Novoa, Eva Maria; Pavon-Eternod, Mariana; Pan, Tao; Ribas de Pouplana, Lluís (March 2012). "A Role for tRNA Modifications in Genome Structure and Codon Usage". Cell. 149 (1): 202–213. doi:10.1016/j.cell.2012.01.050. PMID 22464330. Retrieved 23 November 2014.
- Gebetsberger Jennifer; et al. (2013). "Slicing tRNAs to boost functional ncRNA diversity". RNA biology. 10: 1798–1806. doi:10.4161/rna.27177. PMC . PMID 24351723.
- Shigematsu Megumi; et al. (2014). "Tranfer RNA as a source of small functional RNA". Journal of Molecular Biology and Molecular Imaging. 1: 8.
- Sobala Andrew; et al. (2011). "Transfer RNA-derived fragments: origins, processing, and functions". Wiley interdisciplinary reviews. 2: 853–862. doi:10.1002/wrna.96. PMID 21976287.
- Keam Simon P; et al. (2015). "tRNA-Derived Fragments (tRFs): Emerging New Roles for an Ancient RNA in the Regulation of Gene Expression". Life (Basel). 5: 1638–1651. doi:10.3390/life5041638. PMC . PMID 26703738.
- Telonis Aristeidis G; et al. (2015). "Dissecting tRNA-derived fragment complexities using personalized transcriptomes reveals novel fragment classes and unexpected dependencies". Oncotarget. 6: 24797–822. doi:10.18632/oncotarget.4695. PMC . PMID 26325506.
- Kumar Pankaj; et al. (2014). "Meta-analysis of tRNA derived RNA fragments reveals that they are evolutionarily conserved and associate with AGO proteins to recognize specific RNA targets". BMC Biol. 12: 78. doi:10.1186/s12915-014-0078-0. PMC . PMID 25270025.
- Honda Shozo; et al. (2015). "Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers". Proc Natl Acad Sci USA. 112: E3816–25. doi:10.1073/pnas.1510077112. PMC . PMID 26124144.
- Shigematsu Megumi; et al. (2015). "tRNA-Derived Short Non-coding RNA as Interacting Partners of Argonaute Proteins". Gene Regul Syst Bio. 9: 27–33. doi:10.4137/GRSB.S29411. PMC . PMID 26401098.
- Emara Mohamed M; et al. (2010). "Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly". J Biol Chem. 285: 10959–68. doi:10.1074/jbc.M109.077560. PMC . PMID 20129916.
- Goodarzi Hani; et al. (2015). "Endogenous tRNA-derived fragments suppress breast cancer progression via YBX1 displacement". Cell. 161: 790–802. doi:10.1016/j.cell.2015.02.053. PMC . PMID 25957686.
- Ivanov Pavel; et al. (2011). "Angiogenin-induced tRNA fragments inhibit translation initiation". Mol Cell. 43: 613–23. doi:10.1016/j.molcel.2011.06.022. PMC . PMID 21855800.
- Selitsky Sara R; et al. (2015). "Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C". Sci Rep. 5: 7675. doi:10.1038/srep07675. PMC . PMID 25567797.
- Sharma Upasna; et al. (2016). "Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals". Science. 351: 391–6. doi:10.1126/science.aad6780. PMC . PMID 26721685.
- Casas Eduardo; et al. (2015). "Characterization of circulating transfer RNA-derived RNA fragments in cattle". Front Genet. 6: 271. doi:10.3389/fgene.2015.00271. PMC . PMID 26379699.
- Hirose Yuka; et al. (2015). "Precise mapping and dynamics of tRNA-derived fragments (tRFs) in the development of Triops cancriformis (tadpole shrimp)". BMC Genet. 16: 83. doi:10.1186/s12863-015-0245-5. PMC . PMID 26168920.
- Karaiskos Spyros; et al. (2015). "Age-driven modulation of tRNA-derived fragments in Drosophila and their potential targets". Biol Direct. 10: 51. doi:10.1186/s13062-015-0081-6. PMC . PMID 26374501.
- Pliatsika Venetia; et al. (2016). "MINTbase: a framework for the interactive exploration of mitochondrial and nuclear tRNA fragments". Bioinformatics.
- Kumar Panjav; et al. (2014). "tRFdb: a database for transfer RNA fragments". Nucleic Acids Res. 43: D141–D145. doi:10.1093/nar/gku1138. PMC . PMID 25392422.
- White RJ (1997). "Regulation of RNA polymerases I and III by the retinoblastoma protein: a mechanism for growth control?". Trends in Biochemical Sciences. 22 (3): 77–80. doi:10.1016/S0968-0004(96)10067-0. PMID 9066256.
- Sharp, Stephen; Dingermann, Theodor; Söll, Dieter (1982). "The minimum intragenic sequences required for promotion of eukaryotic tRNA gene transcription" (PDF). Nucleic Acids Research. 10 (18): 5393–5406. doi:10.1093/nar/10.18.5393. PMC . PMID 6924209. Retrieved 23 November 2014.
- Dieci G, Fiorino G, Castelnuovo M, Teichmann M, Pagano A; Fiorino; Castelnuovo; Teichmann; Pagano (December 2007). "The expanding RNA polymerase III transcriptome". Trends Genet. 23 (12): 614–22. doi:10.1016/j.tig.2007.09.001. PMID 17977614.
- Tocchini-Valentini, Giuseppe D.; Fruscoloni, Paolo; Tocchini-Valentini, Glauco P. (12 November 2009). "Processing of multiple-intron-containing pretRNA". Proceedings of the National Academy of Sciences. 106 (48): 20246–20251. doi:10.1073/pnas.0911658106. PMC . PMID 19910528.
- Abelson J, Trotta CR, Li H; Trotta; Li (1998). "tRNA Splicing". J Biol Chem. 273 (21): 12685–12688. doi:10.1074/jbc.273.21.12685. PMID 9582290.
- Soma, Akiko (2014). "Circularly permuted tRNA genes: their expression and implications for their physiological relevance and development". Frontiers in Genetics. 5. doi:10.3389/fgene.2014.00063. ISSN 1664-8021.
- Frank DN, Pace NR; Pace (1998). "Ribonuclease P: unity and diversity in a tRNA processing ribozyme". Annu. Rev. Biochem. 67 (1): 153–80. doi:10.1146/annurev.biochem.67.1.153. PMID 9759486.
- Ceballos M, Vioque A; Vioque (2007). "tRNase Z". Protein Pept. Lett. 14 (2): 137–45. doi:10.2174/092986607779816050. PMID 17305600.
- Randau L, Schröder I, Söll D; Schröder; Söll (May 2008). "Life without RNase P". Nature. 453 (7191): 120–3. Bibcode:2008Natur.453..120R. doi:10.1038/nature06833. PMID 18451863.
- Weiner AM (October 2004). "tRNA maturation: RNA polymerization without a nucleic acid template". Curr. Biol. 14 (20): R883–5. doi:10.1016/j.cub.2004.09.069. PMID 15498478.
- Kutay, U. .; Lipowsky, G. .; Izaurralde, E. .; Bischoff, F. .; Schwarzmaier, P. .; Hartmann, E. .; Görlich, D. . (1998). "Identification of a tRNA-Specific Nuclear Export Receptor". Molecular Cell. 1 (3): 359–369. doi:10.1016/S1097-2765(00)80036-2. PMID 9660920.
- Arts, G. J.; Fornerod, M. .; Mattaj, L. W. (1998). "Identification of a nuclear export receptor for tRNA". Current Biology. 8 (6): 305–314. doi:10.1016/S0960-9822(98)70130-7. PMID 9512417.
- Arts, G. -J.; Kuersten, S.; Romby, P.; Ehresmann, B.; Mattaj, I. W. (1998). "The role of exportin-t in selective nuclear export of mature tRNAs". The EMBO Journal. 17 (24): 7430–7441. doi:10.1093/emboj/17.24.7430. PMC . PMID 9857198.
- Yoshihisa, T.; Yunoki-Esaki, K.; Ohshima, C.; Tanaka, N.; Endo, T. (2003). "Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria". Molecular Biology of the Cell. 14 (8): 3266–3279. doi:10.1091/mbc.E02-11-0757. PMC . PMID 12925762.
- Brian F.C. Clark (October 2006). "The crystal structure of tRNA" (PDF). J. Biosci. 31 (4): 453–7. doi:10.1007/BF02705184. PMID 17206065.
- HOLLEY RW; APGAR J; EVERETT GA; et al. (March 1965). "STRUCTURE OF A RIBONUCLEIC ACID". Science. 147 (3664): 1462–5. Bibcode:1965Sci...147.1462H. doi:10.1126/science.147.3664.1462. PMID 14263761. Retrieved 2010-09-03.
- "The Nobel Prize in Physiology or Medicine 1968". Nobel Foundation. Retrieved 2007-07-28.
- Ladner JE; Jack A; Robertus JD; et al. (November 1975). "Structure of yeast phenylalanine transfer RNA at 2.5 A resolution". Proc. Natl. Acad. Sci. U.S.A. 72 (11): 4414–8. Bibcode:1975PNAS...72.4414L. doi:10.1073/pnas.72.11.4414. PMC . PMID 1105583.
- Kim SH; Quigley GJ; Suddath FL; et al. (1973). "Three-dimensional structure of yeast phenylalanine transfer RNA: folding of the polynucleotide chain". Science. 179 (4070): 285–8. Bibcode:1973Sci...179..285K. doi:10.1126/science.179.4070.285. PMID 4566654.
|Wikimedia Commons has media related to TRNA.|
- tRNAdb (updated and completely restructured version of Spritzls tRNA compilation)
- original Sprinzl tRNA compilation
- tRNA link to heart disease and stroke
- GtRNAdb: Collection of tRNAs identified from complete genomes
- HGNC: Gene nomenclature of human tRNAs
- Molecule of the Month © RCSB Protein Data Bank:
- Rfam entry for tRNA