Taipoxin

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Taipoxin is a potent myo- and neurotoxin, which was isolated from the venom of the coastal taipan Oxyuranus scutellatus or also known as the common taipan.[1] Taipoxin like many other pre-synaptic neurotoxins are phospholipase A2 (PLA2) toxins, which inhibit/complete block the release of the motor transmitter acetylcholine and lead to death by paralysis of the respiratory muscles (asphyxia).[2] It is the most lethal neurotoxin isolated from any snake venom to date.

The molecular mass of the heterotrimer is about 46,000 Dalton; comprising 1:1:1 α, β and γ monomers.[3] Median lethal dose (LD50) for mice is around 1–2 μg/kg (subcutaneous injection).[4][1]

History[edit]

Taipoxin and other PLA2 toxins, have evolved from the digestive PLA2 enzymes.[5] The venom still functions with the almost identical multi-disulphide-bridged protein PLA2 scaffold, which causes the hydrolytic mechanism of the enzyme.[6] However it is thought that under strict evolution selection pressures of prey immobilisation and therefore extended feeding lead to the PLA2 enzyme losing its so called pancreatic loop and mutations for the toxin binding with pre-synaptic membranes of motor neuron end plates.[7][8][9]

Structure[edit]

Taipoxin is a ternary complex consisting of three subunits of α, β and γ monomers in a 1:1:1 ratio, also called the A, B and C homologous subunits.[6] These subunits are equally distributed across the structure and together the three-dimensional structures of these three monomers form a shared core of three α helix's, a Ca2+ binding site and a hydrophobic channel to which the fatty acyl chains binds.[7]

The α and β complex consist of 120 amino acid residues which are cross linked by 7 disulfide bridges. The alpha subunit is very basic (pH(I)>10) and the only one that shows neurotoxicity. The β complex is neutral and can be separated into two isoforms. β1 and β2 are interchangeable but differ slightly in amino acid composition. The γ complex contains 135 amino acid residues which are cross linked by 8 disulfide bridges. It is very acidic due to 4 sialic acid residues, which might be important for complex formation. The gamma subunit also seems to function as a protector of the alpha complex, preventing fast renal clearance or proteolytic degradation. It also boosts the specificity on the target and could be involved in the binding of the alpha unit.[10] The whole complex is slightly acidic with a pH(I) of 5, but under a lower pH and/or high ionic strength the subunits dissociate.

Just as the PLA2 enzyme the PLA2 toxin is Ca2+ dependent for hydrolysing fatty acyl ester bonds at the sn-2 position of glycerol-phospholipids.[7] Depending on disulphide bridge positions and lengths of C-termini these PLA2 enzymes/PLA2 toxins are categorized into three classes. These classes are also an indication of the toxicity of PLA2/PLA2, as PLA2s from pancreatic secretions, bee venom or the weak elapid venoms are grouped into class I, whereas PLA2s from the more potent viperid venoms which causes inflammatory exudate's are grouped into class II. However most snake venoms are capable of more than one toxic activity, such as cytotoxicity, myotoxicity, neuro-toxicity, anticoagulant activity and hypotensive effects.[11][12]

Isolation process[edit]

Taipoxin can be purified from the venom of the coastal taipan by gel filtration chromatography.[1] In addition to taipoxin, the venom consists of many different components, responsible for the complex symptoms.[13]

Mechanism of action[edit]

In the beginning Taipoxin was thought to be only neurotoxic. Studies showed an increase in acetylcholine release, indicating a presynaptic activity.[1] Further experiments showed that Taipoxin inhibited the responses to electrical stimuli greater than the reaction to additionally administered acetylcholine. This led to the conclusion that Taipoxin has pre- and postsynaptic effects. Additional to the increased acetylcholine release it inhibits the vesicular recycling.[14] More recent studies showed that the toxin has a myotoxic effect as well. The injection of Taipoxin into the hind limbs of rats leads to oedema formation and muscle degeneration.[15] The study also supports the findings by Fohlman,[1] that the α subunit yields the PLA2 potency, which is similar to the potency of notexin.[16] Even so, the full potential of the raw toxin is only reached by the combination of the α and γ subunits.[15]

A similar experiment[17] has been done refocusing on the neural compounds. 24 hours after the injection the innervation was compromised to the extent of being unable to identify intact axons. This showed that Taipoxin like toxins lead to the depletion of transmitters from the nerve terminals and lead to the degeneration of nerve terminal and intramuscular axons.[18] In chromaffin cells taipoxin showed the ability to enter the cells via Ca2+ independent mechanisms. There it enhanced catecholamine release in depolarizing cells by disassembling F-actin in the cytoskeletal barrier. This could lead to a vesicle redistribution promoting immediate access into the subplasmalemmal area.[19]

More research studies have found potential binding partners of Taipoxin, which would give more insight into how Taipoxin is transported to the nerve terminals and intramuscular axons.[20][21]

Toxicity[edit]

The toxicity of Taipoxin or other PLA2 toxins are often measured with their ability to cut short chain phospholipids or phospholipids-analogues.[22] For Taipoxin PLA2 activity was set on 0.4 mmol/min/mg, and the binding constant (K) of taipoxin would be equal to: KTaipoxin = KA + KB + KC as it consist out of 3 enzymatic domains/subunits.[6] However no correlation was made between PLA2 activity and toxicity, as the pharmacokinetics and the membrane binding properties are more important. A more specific membrane binding would lead to accumulation of taipoxin in the plasma membranes of motor-neurons.[23][24][25]

Treatment[edit]

The treatment of choice is an antivenom produced by CSL Ltd in 1956 in Australia on the basis of immunised horse plasma.[26] After being bitten the majority of patients will develop systemic envenoming of which clinical evidence is usually present within two hours. This effect can be delayed by applying first aid measures, like immobilization.[13] Additional to neurotoxins taipan venom contains anticoagulants whose effect is also inhibited by the antivenom.

Similar toxins[edit]

Similar to Taipoxin are toxins with different subunits of the PLA domains:

Notexin is a monomer from Notechis scutatus venom, β-bungarotoxin is a heterodimer from Chinese banded krait (Bungarus multicinctus) venom, and Textilotoxin is a pentamer from eastern Pseudonaja textilis venom.

References[edit]

  1. ^ a b c d e Fohlman, J.; Eaker, D.; Karlsson, E.; Thesleff, S. (1976). "Taipoxin, an Extremely Potent Presynaptic Neurotoxin from the Venom of the Australian Snake Taipan (Oxyuranus s. scutellatus). Isolation, Characterization, Quaternary Structure and Pharmacological Properties". European Journal of Biochemistry. 68 (2): 457–469. doi:10.1111/j.1432-1033.1976.tb10833.x. 
  2. ^ Silva, A.; Hodgson, W.; Isbister, G. (2016). "Cross-Neutralisation of In Vitro Neurotoxicity of Asian and Australian Snake Neurotoxins and Venoms by Different Antivenoms". Toxins. 8 (10): 302. doi:10.3390/toxins8100302. 
  3. ^ Alomone labs: Taipoxin (pdf)
  4. ^ Rossetto, O.; Morbiato, L.; Caccin, P.; Rigoni, M.; Montecucco, C. (2006). "Presynaptic enzymatic neurotoxins". Journal of Neurochemistry. 97 (6): 1534–45. doi:10.1111/j.1471-4159.2006.03965.x. PMID 16805767. 
  5. ^ Davidson, F. F.; Dennis, E. A. (1990). "Evolutionary relationships and implications for the regulation of phospholipase A2 from snake venom to human secreted forms". J. Mol. Evol. 31 (3): 228–238. doi:10.1007/BF02109500. PMID 2120459. 
  6. ^ a b c Montecucco, C.; Rossetto, O. (2008). "On the quaternary structure of taipoxin and textilotoxin: The advantage of being multiple". Toxicon. 51 (8): 1560–1562. doi:10.1016/j.toxicon.2008.03.020. PMID 18471843. 
  7. ^ a b c Alape-Girón, Alberto; Persson, Bengt; Cederlund, Ella; Flores-Díaz, Marietta; Gutiérrez, José María; Thelestam, Monica; Bergman, Tomas; Jörnvall, Hans (1999). "Elapid venom toxins: multiple recruitments of ancient scaffolds". European Journal of Biochemistry. 259 (1–2): 225–234. doi:10.1046/j.1432-1327.1999.00021.x. 
  8. ^ Kini, R. M. (1997). Venom Phospholipase A2 Enzymes. Chichester: Wiley. ISBN 0471961892. 
  9. ^ Fletcher, Jeffrey E.; Jiang, Ming-Shi (1995). "Presynaptically acting snake venom phospholipase A2 enzymes attack unique substrates". Toxicon. 33 (12): 1565–1576. doi:10.1016/0041-0101(95)00108-5. 
  10. ^ Fohlman, J.; Lind, P.; Eaker, D. (1977). "Taipoxin, an extremely potent presynaptic snake venom neurotoxin Elucidation of the primary structure of the acidic carbohydrate-containing taipoxin-subunit, a prophospholipase homolog". FEBS Letters. 84 (2): 367–371. doi:10.1016/0014-5793(77)80726-6. 
  11. ^ Lomonte, B.; Tarkowski, A.; Hanson, L. A. (1994). "Broad cytolytic specificity of myotoxin II, a lysine-49 phospholipase A2 of Bothrops asper snake venom". Toxicon. 32 (11): 1359–69. PMID 7886694. 
  12. ^ Gutiérrez, J. M.; Lomonte, B. (1995). "Phospholipase A2 myotoxins from Bothrops snake venoms". Toxicon. 33 (11): 1405–24. PMID 8744981. 
  13. ^ a b "Taipan Antivenom". www.csl.com.au. Retrieved 2017-03-17. 
  14. ^ Hyatt, Mark C.; Russell, James A. (1981). "Effects of β-bungarotoxin and taipoxin on contractions of canine airways caused by nerve stimulation". Life Sciences. 29 (17): 1755–1759. doi:10.1016/0024-3205(81)90185-5. 
  15. ^ a b Harris, J. B.; Maltin, C. A. (1982). "Myotoxic activity of the crude venom and the principal neurotoxin, taipoxin, of the Australian taipan, Oxyuranus scutellatus". British Journal of Pharmacology. 76 (1): 61–75. PMC 2068749Freely accessible. PMID 7082907. 
  16. ^ Harris, J. B.; MacDonell, C. A. (1981-01-01). "Phospholipase A2 activity of notexin and its role in muscle damage". Toxicon. 19 (3): 419–430. PMID 7245222. 
  17. ^ Dixon, R.; Harris, J. B. (1999). "Neurotoxic activity of the toxic phospholipase A2, b-bungarotoxin: Its clinical significance". Am. J. Pathol. 154: 447–455. 
  18. ^ Harris, J. B.; Grubb, B. D.; Maltin, C. A.; Dixon, R. (2000-02-01). "The neurotoxicity of the venom phospholipases A(2), notexin and taipoxin". Experimental Neurology. 161 (2): 517–526. doi:10.1006/exnr.1999.7275. PMID 10686073. 
  19. ^ Ñeco, Patricia; Rossetto, Ornella; Gil, Anabel; Montecucco, Cesare; Gutiérrez, Luis M. (2003-04-01). "Taipoxin induces F-actin fragmentation and enhances release of catecholamines in bovine chromaffin cells". Journal of Neurochemistry. 85 (2): 329–337. doi:10.1046/j.1471-4159.2003.01682.x. 
  20. ^ Kirkpatrick, Laura L.; Matzuk, Martin M.; Dodds, D'Nette C.; Perin, Mark S. (9 June 2000). "Biochemical Interactions of the Neuronal Pentraxins". Journal of Biological Chemistry. 275 (23): 17786–17792. doi:10.1074/jbc.M002254200. 
  21. ^ Dodds, D’Nette C.; Omeis, Ibrahim A.; Cushman, Susan J.; Helms, Jill A.; Perin, Mark S. (22 August 1997). "Neuronal Pentraxin Receptor, a Novel Putative Integral Membrane Pentraxin That Interacts with Neuronal Pentraxin 1 and 2 and Taipoxin-associated Calcium-binding Protein 49". Journal of Biological Chemistry. 272 (34): 21488–21494. doi:10.1074/jbc.272.34.21488. 
  22. ^ Leslie, C. C.; Gelb, M. H (2004). "Assaying phospholipase A2 activity". Methods Mol. Biol.: 229–242. 
  23. ^ Rigoni, M. (2005). "Equivalent Effects of Snake PLA2 Neurotoxins and Lysophospholipid-Fatty Acid Mixtures". Science. 310 (5754): 1678–1680. Bibcode:2005Sci...310.1678R. CiteSeerX 10.1.1.817.8280Freely accessible. doi:10.1126/science.1120640. JSTOR 3842969. PMID 16339444. 
  24. ^ Caccin, Paola; Rigoni, Michela; Bisceglie, Alessandra; Rossetto, Ornella; Montecucco, Cesare (2006). "Reversible skeletal neuromuscular paralysis induced by different lysophospholipids". FEBS Letters. 580 (27): 6317–6321. doi:10.1016/j.febslet.2006.10.039. 
  25. ^ Megighian, Aram; Rigoni, Michela; Caccin, Paola; Zordan, Mauro A.; Montecucco, Cesare (2007). "Alysolecithin/fatty acid mixture promotes and then blocks neurotransmitter release at the Drosophila melanogaster larval neuromuscular junction". Neuroscience Letters. 416 (1): 6–11. doi:10.1016/j.neulet.2007.01.040. PMID 17293048. 
  26. ^ Kuruppu, Sanjaya; Chaisakul, Janeyuth; Smith, A. Ian; Hodgson, Wayne C. (2014-04-01). "Inhibition of Presynaptic Neurotoxins in Taipan Venom by Suramin". Neurotoxicity Research. 25 (3): 305–310. doi:10.1007/s12640-013-9426-z.