The stories are presented in chronological order and offer snapshots of Slayers throughout history, from The First Slayer to Melaka Fray (the Slayer of the future who starred in her own limited comic series). The overarching theme of the graphic novel is on the loneliness and duties of being the Slayer. This is reflected in the juxtaposition of the first line in the first short story ("I am alone"), and the final line in the last ("I am not alone").
A Slayer operating within a walled medieval town during a period of witch-hunts. The Slayer heroically fends off a large-scale attack of vampires, but is burned at the stake afterwards by the very people she saved. Her Watcher, a friar, can do nothing but watch. The story ends as the Watcher lets the vampires into the town, which has no one left to save it.
Fray discovers a former Watcher’s sanctuary and records of her Slayer forebears in 23rd century New York, realizing that while she is the only one in the world, she is not alone. When Fray is next seen in "Time of Your Life" (Season Eight), the Sanctuary is depicted as Fray's main base of operations.