Talk:Expressed sequence tag

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It seems to me that DNA microarrays are used EVALUATE COMPARATIVE diffences in gene TRANSCRIPTION LEVELS, and not to DETERMINE GENE EXPRESSION. The nuance may be subtle... Also, it would be a good idea to explain from where and when a value of 6 million known ESTs as been approximated.

Another topic of interest : Being derived from mRNAs by retro-transcription, are poly-A primers used or random ones? Does it mean that there can be introns in the sequences (as if the cDNAs were obtained from non-spliced mRNAs) as well as 3' and 5' untranslated regions?

Very good article. -Marooned_Morlock


Patents on EST's[edit]

Does anyone have any thoughts on adding to this article information on patent requests on EST's by various BioTech companies? I do recall a story done on a major TV newsmagazine, possibly 20/20 or 60 Minutes, but it was some time ago and I haven't heard much from the mainstream news recently on this. Google produces a significant number of results for the search +patent +gene, but is there enough referenceable info to warrant a separate article for this topic? Ipso-De-Facto 02:34, 9 October 2006 (UTC)

???[edit]

If you don't know the technique, you won't understand it by reading this article. --85.179.34.104 (talk) 11:09, 24 September 2008 (UTC)

Page needs updating[edit]

There's still a section stating "as of 2006", surely this means the page needs a freshening up? — Preceding unsigned comment added by Louismaddox (talkcontribs) 05:07, 13 January 2015 (UTC)