Talk:Isotopic labeling

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Comments from Synthetic Circuits (M.Kang) for ENCH468M[edit]

Summary The article summarizes the use of stable isotope labeling as a method for flux quantification. It introduces a model network and diagrams the passage of mixtures of carbon isotopes (specifically glucose conversion) through the pentose phosphate pathway.

Major Comments: The article needs a more complete introduction of carbon isotopes and their chemical background, possibly in the form of internal links to other Wikipedia pages and a short exposition on the background of metabolic fluxes. The rest of the article talks about isotopes in some degree, but the article itself concerns itself only with biological functions – it needs to be integrated better. The claims made in the article also need sources – these are easy in Wikipedia by simply writing [1] in the page to automatically format endnotes.

Minor Comments: Some cleanup needs to be done, such as properly captioning the images, and capitalizing words unnecessarily, but the content in general seems to be complete. Tagelied (talk) 22:29, 30 November 2011 (UTC)

Comments form group W[edit]

Majors problems: The thing that stuck out was the captions for figures 1 and 2. The figures were good but captions were out of order and did not indicate that the circles represented certain carbon atoms. Words such as NMR, MS, Proton NMR, isotopomer and GC-MS should be briefly explained. It is better to be careful about using abbreviations. “Proton NMR was the first technique that was used for 13C-labeling experiments.” Citations are needed especially the testing isotopes part.

Some points that might help:

You could reduce the description of the various ways to measure isotope labeling, such as radioisotopes labelling and GC-MS etc. You should mention them, but just shorten their description. This article needs a clear explanation of stable isotope labeling and how isotopes are incorporated. The terms such as F6P,T3P,G6P, 2,3-13C T3P should be expanded for someone who is not in this class. It has also some parts that should be put together (Metabolic flux analysis, isotope tracer).“Metabolic pathway analysis … to figuring out the metabolic pathways and reactions that occur within a cell.” Instead, figuring out metabolic pathways that occur within a cell makes sense. In the 'metabolic pathways analysis' part, explain how successful stable isotope labeling is used to elucidate metabolic pathways by giving examples from published papers. You might want to focus more on isotopomer balances (the class note and handout might be helpful), applications to metabolic engineering, possible examples of successful isotope labeling in metabolic engineering. You should be careful about terms that you introduce without giving inadequate explanation. I am not sure if proteomics is a branch of genetics. The 'other use of metabolic engineering' part needs some work.There are some wiki articles that explain some terms in that part. — Preceding unsigned comment added by (talk) 06:48, 1 December 2011 (UTC)

Instructor review

In general, you need to cite more references and at more places than what you have done.

Your article needs hyperlinks to other Wikipedia articles. I am sure there are Wikipedia articles on NMR, MS, etc. that you can link to.

The title "Testing the isotopes" is a little vague. Be more specific.

The sentence beginning with "13C NMR spectrum allows a more detailed isotopomer distribution" is hyperspecialized and hard to follow without a figure. Don't draw a figure, but condense it and make it more lucid so that the message comes across.

There are myriad methods to derivatize compounds for MS. You should mention the ones you list as examples.

Figure numbers are not used in Wikipedia (as far as I know). See Wikipedia featured articles to get an example.

Don't italicize and capitalize when not necessary. For example the expansions of acronyms do not have to be capitalized: write "nuclear magnetic resonance (NMR)" and not "Nuclear Magnetic Resonance (NMR)".

Don't use jargon: "carbon atom", not "carbon"

Don't write directly to the reader, as in "let us assume ..."

The expressions "meaning that" and "last but not least" seems a little awkward. Reword.

UM BIOE120 Instructor (talk) 05:27, 14 December 2011 (UTC)

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  1. ^ Voight, C.A. “Edge Detetction in…” Cell 12, 29 May 2000