|WikiProject Genetics||(Rated C-class, High-importance)|
|WikiProject Molecular and Cell Biology||(Rated B-class, Mid-importance)|
map based cloning
Map based cloning of genes responsible for interesting traits requires local marker saturation around the target gene. This target marker saturation is usually achieved with bulked segregant analysis (Michelmore et al. 1991. PNAS USA 88:9828). --Dan|(talk) 15:00, 6 August 2008 (UTC)
- Tanksley et al. 1995. Trends Genet. 11:63. Chromosome landing: a paradigm for map-based gene cloning in plants with large genomes.
History of cloning doesn't belong here
The section 'history of cloning' is a history of all types of cloning in general, and does not belong in the Molecular Cloning article, but rather in the Cloning article. It is also cited incorrectly. Greeney (talk) 05:31, 23 August 2008 (UTC)
Definitions needed . . .
Contradiction: Transformation: "the process in which cells take up DNA directly from their environment" [Campbell, Reece and Meyers, 2009]
The comment(s) below were originally left at several discussions in past years, these subpages are now deprecated. The comments may be irrelevant or outdated; if so, please feel free to remove this section., and are posted here for posterity. Following
|Hey. I read this page, and I think it needs attention. In general the explanation for molecular cloning was somewhat vague, and it was difficult to follow in general. I think this page should also have a picture to better explain. A picture of the steps will go a very long way with this article.|
Last edited at 21:20, 18 February 2008 (UTC). Substituted at 00:19, 30 April 2016 (UTC)