Talk:Recombinase Polymerase Amplification

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Reads like an advertisement[edit]

I've never used RPA myself, but this wiki entry reads like PR literature put out by the manufacturer (TwistDx). I don't doubt that the technique has been demonstrated to perform well for certain applications, and has certain inherent advantages over PCR and maybe has certain advantages over other isothermal techniques (e.g. LAMP, NASBA etc) but it surely has some drawbacks as well. To wit the primer design rules are not fully understood (per the manufacturer's own FAQ), the probe chemistry is proprietary, and the kit contents are not fully disclosed so you have to buy the kit at high price from the manufacturer, whereas anyone (for research purposes) can mix their own reagents for PCR, LAMP, etc. To my knowledge there is not much in the scientific literature that systematically evaluates the relative merits of these techniques - I'm sure the companies that own the IP for HDA, SDA, NEAR/DNAble etc would be happy to point out articles demonstrating the superiority of their techniques as well. I myself only have detailed knowledge of a couple of these techniques so I'm not the right person to add some levity to this article, but maybe someone else would know more. — Preceding unsigned comment added by RobertM75 (talkcontribs) 18:40, 14 May 2014 (UTC)