Test tube

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Test tube
Two small test tubes held in spring clamps
Other names Culture tube
Uses Chemical reaction
Related items Vacutainer
Boiling tube
Centrifuge tube

A test tube, also known as a culture tube or sample tube, is a common piece of laboratory glassware consisting of a finger-like length of glass or clear plastic tubing, open at the top, usually with a rounded U-shaped bottom.

A large test tube specifically for boiling liquids is called a boiling tube.

Test tubes are available in a multitude of lengths and widths, typically from 10 to 20 mm wide and 50 to 200 mm long.[1] The top often features a flared lip to aid pouring out the contents; some sources consider that the presence of a lip is what distinguishes a test tube from a culture tube.[2] A test tube has either a flat bottom, a round bottom, or a conical bottom. Some test tubes are made to accept a ground glass stopper or a screw cap. They are often provided with a small ground glass or white glaze area near the top for labelling with a pencil.


Test tubes are widely used by chemists to handle chemicals, especially for qualitative experiments and assays. Their spherical bottom and vertical sides reduce mass loss when pouring, make them easier to wash out, and allow convenient monitoring of the insides. The long, narrow neck slows down the spreading of vapours and gases to the environment.

A test tube filled with water and upturned into a water-filled beaker is often used to capture gases, e.g. in electrolysis demonstrations.

Samples of human blood collected for blood tests.

Culture tubes are often used in biology for handling and culturing all kinds of live organisms, such as molds, bacteria, seedlings, plant cuttings, etc.; and in medicine and forensics to store samples of blood or other fluids.

A test tube with a stopper is often used for temporary storage of chemical or biological samples.

Test tubes are usually held in special-purpose racks, clamps, or tongs. Some racks for culture tubes are designed to hold the tubes in a nearly horizontal position, so as to maximize the surface of the culture medium inside.

Test tubes are sometimes put to casual uses outside of lab environments, e.g. as flower vases, glassware for certain weak shots, or containers for spices.

Where large numbers of tests are run or only small amounts are available for testing, or both, microtiter plates are often used as small test tubes.


Eight cavity test tube mold courtesy of Lake Charles Manufacturing.

Test tubes for physics and chemistry are usually made of glass for better resistance to heat and corrosive chemicals and longer life. Tubes made from expansion-resistant glasses, mostly borosilicate glass (or fused quartz), can be placed directly over a Bunsen burner flame.

Culture tubes for biology are usually made of clear plastic (such as polystyrene or polypropylene) by injection molding [3] and are often discarded after use. Plastic test tubes with a screwtop cap are often called 'Falcon tubes' after a line manufactured by Becton Dickinson.[4]

Test tubes may come with prepared contents. For example, a blue top tube is a test tube of 5 ml containing sodium citrate as an anticoagulant, used to collect specimens for coagulation screens and testing for glucose-6-phosphate dehydrogenase.[5] A test tube is very resistant and can usually withstand temperatures over 300 degrees C.


Main article: Vacutainer

In clinical medicine, test tubes are used to hold samples. Unlike laboratory test tubes, these tubes are commonly sealed with a rubber stopper and have a specific additive placed in the tube with the stopper color indicating the additive. Additionally, these tubes are sterile and have the air removed. Blood is the most obvious use for these tubes but any other bodily fluid (e.g., urine, pus, synovial) can be placed in these tubes.

See also[edit]


  1. ^ MiniScience.com catalog: Test Tube, accessed March 27, 2009
  2. ^ Thomas Scott (transl., 1996), Concise Encyclopedia: Biology. Walter de Gruyter. ISBN 3-11-010661-2, ISBN 978-3-11-010661-9. 1287 pages.
  3. ^ M. Jeremy Ashcraft; General Manager; Lake Charles Manufacturing (2007). Test Tube Molding Process: A discussion on the molding of plastic test tubes. Lake Charles Manufacturing. 
  4. ^ "BD Falcon Tubes and Pipets" (PDF). Becton Dickinson. Retrieved 14 March 2016. 
  5. ^ TheFreeDictionary > blue top tube Citing: McGraw-Hill Concise Dictionary of Modern Medicine. 2002 by The McGraw-Hill Companies, Inc.

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