He was born in London, the son of Thomas Gatacre. He was educated at St John's College, Cambridge. From 1601 to 1611 he held the appointment of preacher to the society of Lincoln's Inn, which he resigned on accepting the rectory of Rotherhithe. In 1642 he was chosen a member of the Westminster Assembly, and annotated for them the books of Isaiah, Jeremiah and Lamentations.
His principal works, besides some volumes of sermons, are:
- Of the Nature and Use of Lots (1619), a curious treatise which led to his being accused of favouring games of chance, but which Boswell called "a learned book of the age". The second edition in 1627 contained a refutation of James Balmford's criticism. It was edited and republished in 2008 as The Nature and Uses of Lotteries. Conall Boyle (ed.) Imprint Academic.
- Dissertatio de stylo Novi Testamenti (1648)
- Cinnus, sive Adversaria miscellanea, in quibus Sacrae Scripturae primo, deinde aliorum scriptorum, locis aliquam multis lux redditur (1651), to which was afterwards subjoined Adversaria Posthuma
- an edition of Meditations by Marcus Aurelius (1652)
His collected works were published in Utrecht in 1698.
- Nomenclator Philologorum by Friedrich August Eckstein
- Stephen, Leslie; Lee, Sidney, eds. (1890). . Dictionary of National Biography. 21. London: Smith, Elder & Co.
- "Thomas Gataker (GTKR593T)". A Cambridge Alumni Database. University of Cambridge.
- "Rotherhithe - British History Online".
- James Boswell (Henry Morley, ed), The life of Samuel Johnson, LL.D. and The Journal of his tour to the Hebrides [Joshua Reynolds Edition], Vol V, Routledge, 1885, p. 228
- discussion in J. Franklin, The Science of Conjecture: Evidence and Probability Before Pascal. Baltimore: Johns Hopkins University Press, 2001. pp. 284–285.
- "Conall Boyle".
- "Imprint Academic - Imprint Academic".
- According to Hallam, is the "earliest edition of any classical writer published in England with original annotations," and, for the period at which it was written, possesses remarkable merit.
- This article incorporates text from a publication now in the public domain: Chisholm, Hugh, ed. (1911). "Gataker, Thomas". Encyclopædia Britannica. 11 (11th ed.). Cambridge University Press. p. 527.