Coordinates: The Totley Brook is a stream in Sheffield, South Yorkshire, England. It rises on a millstone grit ridge some 7 miles (11 km) to the south-west of the centre of Sheffield. Over its course it drops from 740 feet (230 m) to 430 feet (130 m) near its junction with the Old Hay Brook close to Baslow Road. The streams form the River Sheaf once they have joined.
The Totley Brook runs around the south of Totley through Gillfield Woods, flows underneath Mickley Lane, behind the Totley Rise shops and meets Old Hay behind Milldale where Totley Chemical Works once stood. The Works was run by Tinker and Siddall from 1846, and had been taken over by Thomas Kilner by 1899, who manufactured pyroligneous acid, naptha and charcoal.
- Ball Crossley and Flavell, (2006), p. 148
- Ordnance Survey, 1:25000 map, Sheet OL24
- Totley Independent, Issue 317, October 2008
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