Trikolonoi (Greek: Τρικόλωνοι) is a former municipality in Arcadia, Peloponnese, Greece. Since the 2011 local government reform it is part of the municipality Gortynia, of which it is a municipal unit. The municipal unit includes the villages of Stemnitsa (the former municipal seat), Syrna, Pavlia, Palamari and Elliniko. Trikolonoi is located southeast of Dimitsana, west of Tripoli and north of Megalopoli. In 2011 Trikolonoi had a population of 578.
The municipal unit Trikolonoi is subdivided into the following communities (constituent villages in brackets):