Besen formed Truth Wins Out on June 7, 2006 as a response to the belief that a person's homosexual orientation can change. In particular, TWO is critical of organizations and religious ministries which treat homosexuality as a diseased behavior pattern which can be "cured". Besen later launched the related RespectMyResearch project to document what he views as distortions of science by the ex-gay movement, especially Focus on the Family. Former staff include John M. Becker, who served as director of communications and development.
Truth Wins Out organizes protests around the country to counter the ex-gay movement. One of TWO's three largest protests occurred on November 4, 2008 at the National Radio Hall of Fame in Chicago. The "Dobson" campaign was being held to protest the induction of James Dobson, the founder of Focus on the Family, into the Radio Hall of Fame. On his websites, Besen posts videos of former ex-gays describing their experience with the ex-gay movement.