Tsirguliina is a small borough (alevik) in Tõlliste Parish, Valga County in southern Estonia, located about 13 km northeast of the town of Valga. With a population of 499 (as of 2011) it is the most populous settlement in the municipality; the area is 2.57 km². With the most important institutions and facilities like high school, kindergarten, civic centre, library, post office and medical office, it is the unofficial centre of the parish. The local government is located 3 km east in Laatre.
Tsirguliina was established around the Sangaste station on Tartu–Valga railway shortly after its opening in 1887. Nowadays the train station is still used by passenger traffic.