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Tsuneko Okazaki(岡崎 恒子?,Okazaki Tsuneko, born June 7, 1933) is a Japanese scientist known for her discovery and research of Okazaki fragments along with her husband Reiji.
Okazaki was born in the Aichi Prefecture of Japan in 1933. She graduated with her PhD from Nagoya University School of Science in 1956, which was also the year that she met her husband, Reiji Okazaki. In 1968, Reiji and Tsuneko published their breakthrough findings on Okazaki fragments in PNAS.