Tukey's test of additivity
In statistics, Tukey's test of additivity, named for John Tukey, is an approach used in two-way ANOVA (regression analysis involving two qualitative factors) to assess whether the factor variables are additively related to the expected value of the response variable. It can be applied when there are no replicated values in the data set, a situation in which it is impossible to directly estimate a fully general non-additive regression structure and still have information left to estimate the error variance. The test statistic proposed by Tukey has one degree of freedom under the null hypothesis, hence this is often called "Tukey's one-degree-of-freedom test."
The most common setting for Tukey's test of additivity is a two-way factorial Analysis of Variance (ANOVA) with one observation per cell. The response variable Yij is observed in a table of cells with the rows indexed by i = 1,..., m and the columns indexed by j = 1,..., n. The rows and columns typically correspond to various types and levels of treatment that are applied in combination.
The additive model states that the expected response can be expressed EYij = μ + αi + βj, where the αi and βj are unknown constant values. The unknown model parameters are usually estimated as
where Yi• is the mean of the ith row of the data table, Y•j is the mean of the jth column of the data table, and Y•• is the overall mean of the data table.
The additive model can be generalized to allow for arbitrary interaction effects by setting EYij = μ + αi + βj + γij. However, after fitting the natural estimator of γij,
the fitted values
fit the data exactly. Thus there are no remaining degrees of freedom to estimate the variance σ2, and no hypothesis tests about the γij can performed.
Tukey therefore proposed a more constrained interaction model of the form
By testing the null hypothesis that λ = 0, we are able to detect some departures from additivity based only on the single parameter λ.
To carry out Tukey's test, set
Then use the following test statistic 
Under the null hypothesis, the test statistic has an F distribution with 1, q degrees of freedom, where q = mn − (m + n) is the degrees of freedom for estimating the error variance.
- Tukey's range test for multiple comparisons
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