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U3 or U-3 may refer to:
- U3, an underground rapid transit line in many major German/Austrian cities:
- Avies (IATA code: U3), an Estonian airline
- Cessna U-3, the military model of the Cessna 310 aircraft
- German submarine U-3, one of several German submarines
- U3 (software), a design specification for USB storage, created by U3, LLC
- U3, a speed class for Secure Digital (SD) cards
- U3, an unemployment figure released by the United States Bureau of Labor Statistics
- Small nucleolar RNA U3, a type of non-coding RNA
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