Up follows the storyline of the film, featuring Carl, Russell, and Dug walking through the jungles of Venezuela. All of the mentioned characters are playable in the game. The game contains multi-player gameplay and aerial combat in addition to the main game.
The PlayStation 3 port of the game was the first game from Pixar to support the trophy system.
The Mac, PC, PS2, PSP, and DS versions of the game have received an E rating for Cartoon Violence, while the Wii, Xbox 360 and PS3 versions have an E10+ rating for Animated Blood and Mild Cartoon Violence.