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Protein VPS25 PDB 2ZME.png
Available structures
PDB Ortholog search: PDBe RCSB
Aliases VPS25, DERP9, EAP20, FAP20, vacuolar protein sorting 25 homolog
External IDs MGI: 106354 HomoloGene: 6303 GeneCards: VPS25
RNA expression pattern
PBB GE VPS25 gnf1h00737 at fs.png
More reference expression data
Species Human Mouse
RefSeq (mRNA)



RefSeq (protein)


Location (UCSC) Chr 17: 42.77 – 42.78 Mb Chr 11: 101.25 – 101.26 Mb
PubMed search [1] [2]
View/Edit Human View/Edit Mouse
ESCRT-II complex subunit
PDB 1xb4 EBI.jpg
crystal structure of subunit vps25 of the endosomal trafficking complex escrt-ii
Pfam PF05871
InterPro IPR008570

Vacuolar protein-sorting-associated protein 25 is a protein that in humans is encoded by the VPS25 gene.[3][4]

It is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT (ESCRT-I, -II, -III) complexes orchestrate efficient sorting of ubiquitinated transmembrane receptors to lysosomes via multivesicular bodies (MVBs).[5] ESCRT-II recruits the transport machinery for protein sorting at MVB.[6] In addition, the human ESCRT-II has been shown to form a complex with RNA polymerase II elongation factor ELL in order to exert transcriptional control activity. ESCRT-II transiently associates with the endosomal membrane and thereby initiates the formation of ESCRT-III, a membrane-associated protein complex that functions immediately downstream of ESCRT-II during sorting of MVB cargo. ESCRT-II in turn functions downstream of ESCRT-I, a protein complex that binds to ubiquitinated endosomal cargo.[7]

ESCRT-II is a trilobal complex composed of two copies of vps25, one copy of vps22 and the C-terminal region of vps36. The crystal structure of vps25 revealed two winged-helix domains, the N-terminal domain of vps25 interacting with vps22 and vps36.[8]


  1. ^ "Human PubMed Reference:". 
  2. ^ "Mouse PubMed Reference:". 
  3. ^ Yorikawa C, Shibata H, Waguri S, Hatta K, Horii M, Katoh K, Kobayashi T, Uchiyama Y, Maki M (Mar 2005). "Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting". Biochem J. 387 (Pt 1): 17–26. doi:10.1042/BJ20041227. PMC 1134928Freely accessible. PMID 15511219. 
  4. ^ "Entrez Gene: VPS25 vacuolar protein sorting 25 homolog (S. cerevisiae)". 
  5. ^ Gill DJ, Teo H, Sun J, Perisic O, Veprintsev DB, Emr SD, Williams RL (January 2007). "Structural insight into the ESCRT-I/-II link and its role in MVB trafficking". EMBO J. 26 (2): 600–12. doi:10.1038/sj.emboj.7601501. PMC 1783442Freely accessible. PMID 17215868. 
  6. ^ Teo H, Perisic O, Gonzalez B, Williams RL (October 2004). "ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes". Dev. Cell. 7 (4): 559–69. doi:10.1016/j.devcel.2004.09.003. PMID 15469844. 
  7. ^ Babst M, Katzmann DJ, Snyder WB, Wendland B, Emr SD (August 2002). "Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body". Dev. Cell. 3 (2): 283–9. doi:10.1016/S1534-5807(02)00219-8. PMID 12194858. 
  8. ^ Wernimont AK, Weissenhorn W (December 2004). "Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II". BMC Struct. Biol. 4 (1): 10. doi:10.1186/1472-6807-4-10. PMC 539351Freely accessible. PMID 15579210. 

Further reading[edit]

This article incorporates text from the public domain Pfam and InterPro IPR008570