Van den Bergh reaction

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Van den Bergh reaction is a chemical reaction used to measure bilirubin levels in blood.[1] More specifically, it determines the amount of conjugated bilirubin in the blood. The reaction produces azobilirubin. Principle: bilirubin reacts with diazotised sulphanilic acid to produce purple coloured azo bilirubin.[2]


  1. ^ 1939
  2. ^ "BILIRUBIN AND THE VAN DEN BERGH REACTION" (PDF). Biochem Journal. Retrieved February 18, 2014.