Vernalization (from Latin vernus, "of the spring") is the acquisition of a plant's ability to flower in the spring by exposure to the prolonged cold of winter, or by an artificial equivalent. After vernalization, plants have acquired the ability to flower, but they may require additional seasonal cues or weeks of growth before they will actually flower. Vernalization can also refer to herbal (non-woody) plants requiring a cold dormancy to produce new shoots and leaves. 
Many plants grown in temperate climates require vernalization and must experience a period of low winter temperature to initiate or accelerate the flowering process. This ensures that reproductive development and seed production occurs in spring and summer, rather than in autumn. The needed cold is often expressed in chill hours. Typical vernalization temperatures are between 0 and 5 degrees Celsius (40 and 50 degrees Fahrenheit).
For many perennial plants, such as fruit tree species, a period of cold is needed first to induce dormancy and then later, after the requisite period of time, re-emerge from that dormancy prior to flowering. Many monocarpic winter annuals and biennials, including some ecotypes of Arabidopsis thaliana and winter cereals such as wheat, must go through a prolonged period of cold before flowering occurs.
History of vernalization research
In the history of agriculture, farmers observed a traditional distinction between "winter cereals," whose seeds require chilling (to trigger their subsequent emergence and growth), and "spring cereals," whose seeds can be sown in spring, thence they germinate, and then flower soon thereafter. The word "vernalization" is a translation of "яровизация" ("jarovization") a word coined by Trofim Lysenko to describe a chilling process he used to make the seeds of winter cereals behave like spring cereals ("Jarovoe" in Russian). Scientists had also discussed how some plants needed cold temperatures to flower, as early as the 18th century, with the German plant physiologist Gustav Gassner often mentioned for his 1918 paper.
Lysenco's 1928 paper on vernalization and plant physiology drew wide attention due to its practical consequences for Russian agriculture. Severe cold and lack of winter snow had destroyed many early winter wheat seedlings. By treating wheat seeds with moisture as well as cold, Lysenco induced them to bear a crop when planted in spring. Later, however, Lysenco inaccurately asserted that the vernalized state could be inherited - i.e., that the offspring of a vernalized plant would behave as if they themselves had also been vernalized and would not require vernalization in order to flower quickly.
Early research on vernalization focused on plant physiology; the increasing availability of molecular biology has made it possible to unravel its underlying mechanisms. For example, a lengthening daylight period (longer days), as well as cold temperatures are required for winter wheat plants to go from the vegetative to the reproductive state; the three interacting genes are called VRN1, VRN2, and FT (VRN3).
In Arabidopsis thaliana
Arabidopsis thaliana, also known as "thale cress," is a much-studied model species. In 2000, the entire genome of its five chromosomes was completely sequenced. Some variants, called "winter annuals", require vernalization to flower; others ("summer annuals") do not. The genes that underlie this difference in plant physiology have been intensively studied.
The reproductive phase change of A. thalliana occurs by a sequence of two related events: first, the bolting transition (flower stalk elongates), then the floral transition (first flower appears). Bolting is a robust predictor of flower formation, and hence a good indicator for vernalization research.
In arabidopsis winter annuals, the apical meristem is the part of the plant that needs to be chilled. Vernalization of the meristem appears to confer competence to respond to floral inductive signals on the meristem. A vernalized meristem retains competence for as long as 300 days in the absence of an inductive signal.
Before vernalization, flowering is repressed by the action of a gene called Flowering Locus C (FLC). Prolonged exposure to cold induces expression of VERNALIZATION INSENSTIVE3, which interacts with the VERNALIZATION2 polycomb-like complex to reduce FLC expression through chromatin remodeling. The epigenetic silencing of FLC by chromatin remodeling involves down-regulation of FLC expression by cold-induced expression of antisense FLC COOLAIR transcripts.  It is also clear that FLC silencing must be achieved at the whole plant level by an appropriate proportion of individual cells switching into a stable FLC-silenced state.  In order that vernalization is reset in the next generation, FLC repression is removed during embryogenesis by a mechanism involving a H3K27 methylase 
It is possible to devernalize a plant by exposure to high temperatures subsequent to vernalization. For example, commercial onion growers store seeds at low temperatures, but devernalize them before planting, because they want the plant's energy to go into enlarging its bulb (underground stem), not making flowers.
- K. Sokolski, A. Dovholuk, L. Dovholuk and P. Faletra Selbyana Vol. 18, No. 2, ORCHIDS (1997), pp. 172-182
- Sibum Sung; Yuehui He; Tifani W Eshoo; Yosuke Tamada; Lianna Johnson; Kenji Nakahigashi; Koji Goto; Steve E Jacobsen; Richard M Amasino (June 2006). "Epigenetic maintenance of the vernalized state in Arabidopsis thaliana requires LIKE HETEROCHROMATIN PROTEIN 1". Nature Genetics (Nature) 38 (6): 706–710. doi:10.1038/ng1795. PMID 16682972.
- Michaels, Sd; He, Y; Scortecci, Kc; Amasino, Rm (Aug 2003). "Attenuation of FLOWERING LOCUS C activity as a mechanism for the evolution of summer-annual flowering behavior in Arabidopsis." (Free full text). Proceedings of the National Academy of Sciences of the United States of America 100 (17): 10102–7. Bibcode:2003PNAS..10010102M. doi:10.1073/pnas.1531467100. PMC 187779. PMID 12904584
- P. Chouard (June 1960). "Vernalization and its relations to dormancy". Annual Reviews of Plant Physiology (Annual Reviews) 11: 191–238. doi:10.1146/annurev.pp.11.060160.001203. Retrieved 2011-01-25.
- Nils Roll-Hansen (1985). "A new perspective on Lysenko?". Annals of Science (Taylor & Francis) 42 (3): 261–278. doi:10.1080/00033798500200201. PMID 11620694. Retrieved 2015-02-18.
lysencosciwas invoked but never defined (see the help page).
Cite error: The named reference
- Richard Amasino (October 2004). "Vernalization, Competence, and the Epigenetic Memory of Winter". Plant Cell (American Society of Plant Biologists) 16 (10): 2553–2559. doi:10.1105/tpc.104.161070. PMC 520954. PMID 15466409.
- Ben Trevaskis; Megan N. Hemming; Elizabeth S. Dennis (August 2007). "The molecular basis of vernalization-induced flowering in cereals". Trends in Plant Science (Elsevier) 12 (8): 352–357. doi:10.1016/j.tplants.2007.06.010. PMID 17629542.
- "New genetic model predicts plant flowering in different environments". eScienceNews. Retrieved 2011-01-25.
- Walbot, Virginia (2000). "A green chapter in the book of life". Nature 408 (6814): 794–795. doi:10.1038/35048685. PMID 11130710. Retrieved 28 January 2011.
- "Vernalisation response". Plant Biology. Retrieved 2011-01-26.
- Pouteau, Sylvie; Catherine Albertini (2009). "The significance of bolting and floral transitions as indicators of reproductive phase change in Arabidopsis". J. Exp. Bot. 60 (12): 3367–3377. doi:10.1093/jxb/erp173. PMID 19502535. Retrieved 29 January 2011.
- Sung, Sibum; Amasino, Richard M. (2004). "Vernalization in Arabidopsis thaliana is mediated by the PHD finger protein VIN3". Nature 427 (6970): 159–163. Bibcode:2004Natur.427..159S. doi:10.1038/nature02195.
- Tibor Csorba; Julia I. Questa; Qianwen Sun; Caroline Dean (2014). "Antisense COOLAIR mediates the coordinated switching of chromatin states at FLC during vernalization". PNAS USA 111: 16160–5. doi:10.1073/pnas.1419030111.
- Andrew Angel; Jie Song; Caroline Dean; Martin Howard (2011). "A Polycomb-based switch underlying quantitative epigenetic memory". Nature 476: 105–108. doi:10.1038/nature10241.
- Pedro Crevillen; H. Yang; X. Cui; C. Greeff; M. Trick; Q. Qiu; X. Cao; C. Dean (2014). "Epigenetic reprogramming that prevents transgenerational inheritance of the vernalized state". Nature 515: 587–90. doi:10.1038/nature13722.
- "Vernalisation pathway". Plant Biology. Retrieved 2011-01-26.
- "Vernalization". Encyclopædia Britannica Online. Retrieved 2011-01-24.
vernalization, the artificial exposure of plants (or seeds) to low temperatures in order to stimulate flowering or to enhance seed production. By satisfying the cold requirement of many temperate-zone plants, flowering can be induced to occur earlier than normal or in warm climates lacking the requisite seasonal chilling.