The Vesicular acetylcholine transporter (VAChT) is a neurotransmitter transporter which is responsible for loading acetylcholine (ACh) into secretory organelles in neurons making acetylcholine available for secretion. It is encoded by Solute carrier family 18, member 3 (SLC18A3) gene. VAChT is able to transport ACh into vesicles by relying on an exchange between protons that were previously pumped into the vesicle diffusing out. ACh molecules are then carried into the cell by the action of exiting protons.  Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
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