Ambrose was born at Chester, the son of Richard and Mary Ambrose. He was called to the bar at Lincolns Inn in 1859 and migrated to Middle Temple in 1869. In 1874, he became a Queen's Counsel and in 1881 a bencher. At the 1885 general election he was elected as Tory MP for Harrow. He held the seat until 1899 when he resigned on being appointed a Master in Lunacy.
He is buried in Highgate Cemetery, in the West Cemetery, along with his wife and her parents.
- Leigh Rayment's Historical List of MPs – Constituencies beginning with "H" (part 1)
- Holy Trinity Chester - Parish Register
- Debretts House of Commons and Judicial Guide 1886
- Craig, F. W. S. (1989) . British parliamentary election results 1885–1918 (2nd ed.). Chichester: Parliamentary Research Services. p. 347. ISBN 0-900178-27-2.
- British Census 1881 RG11 1367/125 p57
- Hansard 1803–2005: contributions in Parliament by William Ambrose
|Parliament of the United Kingdom|
|New constituency|| Member of Parliament for Harrow
1885 – 1899
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