XLT agar

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XLT Agar (Xylose Lysine Tergitol-4) Is a selective culture medium for the isolation and identification of salmonellae from food and environmental samples. It is similar to XLD agar however the agar is supplemented with the surfactant, Tergitol 4, which causes inhibition of Proteus spp. and other non-Salmonellae.[1] Successful growth of Salmonella will result in growth of red colonies with a black centre.

  • XLT Agar contains:
Proteose Peptone 1.6g/l
Yeast extract 3g/l
L-Lysine 5g/l
Xylose 3.75g/l
Lactose 7.5g/l
Sucrose 7.5g/l
Sodium chloride 5g/l
Sodium thiosulphate 6.8g/l
Ferric ammonium citrate 0.8 mg/l
Phenol red 0.08g/l
Agar 18g/l
[Tergitol-4] 4.6mL/l

See also[edit]


  1. ^ Dusch, H. & Altwegg, M. (1995). "Evaluation of Five New Plating Media for Isolation of Salmonella Species". Journal of Clinical Microbiology. 33 (4): 802–804. PMC 228044. PMID 7790441.

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