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YPD medium bottle and YPD agar plate

YEPD or yeast extract peptone dextrose, also often abbreviated as YPD, is a complete medium for yeast growth. It contains yeast extract, peptone, double-distilled water, and glucose or dextrose.[1] It can be used as solid medium by including agar. The yeast extract will typically contain all the amino acids necessary for growth. By being a complete medium, YEPD cannot be used as a selection medium to test for auxotrophs. Instead, YEPD is used as a growth medium to grow yeast cultures.

The broth version of YEPD typically contains 1% yeast extract, 2% peptone, 2% glucose/dextrose, and the rest is distilled water. While the agar version includes addition of 1.5 to 2% of agar before allowing mixture to cool and solidify.[2][3]


  1. ^ "YPD media". Cold Spring Harbor Protocols. 2010 (9): pdb.rec12315. 2010-09-01. doi:10.1101/pdb.rec12315. ISSN 1940-3402.
  2. ^ "Yeast Extract-Peptone-Dextrose (YPD) Agar" (PDF). Retrieved 2017-12-29.
  3. ^ "protocol: YPD plates". Retrieved 2017-12-29.