Yukiko Yamashita (born 1971) is an American developmental biologist. She is a faculty member of the University of Michigan Life Sciences Institute and associate professor for the Department of Cell and Developmental Biology at the University of Michigan Medical School. She was appointed an HHMI Investigator in 2013. In November 2013 she received a 5-year appointment as the James Playfair McMurrich Collegiate Professor of the Life Sciences at the University of Michigan Medical School.
She received a Keck Foundation Award in 2012. She is a 2011 MacArthur Fellow and a 2008 Searle Scholar.
She graduated from Kyoto University with a BS and PhD in Biophysics, and was a postdoctoral fellow with Margaret T. Fuller at Stanford University from 2001 to 2006.