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Z-line can refer to:
- the borders that separate and link sarcomeres within a skeletal muscle
- a ban on a user's IP address for the purposes of blocking their access to Internet Relay Chat. See Z-line.
- the gastroesophageal junction that joins the esophagus to the stomach.
- Z, a New York City Subway service.
| disambiguation page lists articles associated with the title Z-line.
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