Zerewitinoff determination

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The Zerewitinoff determination or Zerevitinov determination is a quantitative chemical test for the determination of active hydrogens in a chemical substance. A sample is treated with the Grignard reagent, methylmagnesium iodide, which reacts with any acidic hydrogen atom to form methane. This gas can be determined quantitatively. For example:

Zerewitinow reaction.png

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