Mitoplast: Difference between revisions
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A '''mitoplast''' is a [[mitochondrion]] that has been stripped of its [[inner mitochondrial membrane|outer membrane]] leaving the [[Inner mitochondrial membrane|inner membrane]] intact.<ref>{{cite web|title=Mitoplast|url=http://www.genscript.com/product_003/molecular_biology_glossary/id/1892/category/glossary/mitoplast.html|work=Glossary of Biochemistry and Molecular Biology|publisher=GenScript}}</ref> |
A '''mitoplast''' is a [[mitochondrion]] that has been stripped of its [[inner mitochondrial membrane|outer membrane]] leaving the [[Inner mitochondrial membrane|inner membrane]] intact.<ref>{{cite web|title=Mitoplast|url=http://www.genscript.com/product_003/molecular_biology_glossary/id/1892/category/glossary/mitoplast.html|work=Glossary of Biochemistry and Molecular Biology|publisher=GenScript}}</ref> |
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== In Electrophysiology == |
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'''Mitoplasts''' are useful for [[Electrophysiology|electrophysiological]] analysis of mitochondrial function. Specifically, [[Patch clamp|patch-clamp]] electrophysiology has emerged as a novel method for studying functionality of the inner mitochondrial membrane (IMM)<ref>{{Cite journal|last=Bertholet|first=Ambre M.|last2=Kirichok|first2=Yuriy|date=2020|title=Patch-Clamp Analysis of the Mitochondrial H+ Leak in Brown and Beige Fat|url=https://www.frontiersin.org/articles/10.3389/fphys.2020.00326/full|journal=Frontiers in Physiology|language=English|volume=0|doi=10.3389/fphys.2020.00326|issn=1664-042X}}</ref>. This method facilitates sensitive current measurement across the IMM, useful for studying the [[electron transport chain]] and proton leak (i.e., the uncoupling of proton movement down it's [[electrochemical gradient]] and ATP synthesis via [[ATP synthase]]). |
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==References== |
==References== |
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Revision as of 18:29, 10 September 2021
A mitoplast is a mitochondrion that has been stripped of its outer membrane leaving the inner membrane intact.[1]
In Electrophysiology
Mitoplasts are useful for electrophysiological analysis of mitochondrial function. Specifically, patch-clamp electrophysiology has emerged as a novel method for studying functionality of the inner mitochondrial membrane (IMM)[2]. This method facilitates sensitive current measurement across the IMM, useful for studying the electron transport chain and proton leak (i.e., the uncoupling of proton movement down it's electrochemical gradient and ATP synthesis via ATP synthase).
References
- ^ "Mitoplast". Glossary of Biochemistry and Molecular Biology. GenScript.
- ^ Bertholet, Ambre M.; Kirichok, Yuriy (2020). "Patch-Clamp Analysis of the Mitochondrial H+ Leak in Brown and Beige Fat". Frontiers in Physiology. 0. doi:10.3389/fphys.2020.00326. ISSN 1664-042X.
{{cite journal}}: CS1 maint: unflagged free DOI (link)
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