Restriction fragment mass polymorphism: Difference between revisions
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'''Restriction Fragment Mass Polymorphism (RFMP)''' is a technology which [[Restriction digest|digests]] [[DNA]] into [[oligonucleotide]] fragments, and detects variation of [[DNA sequence]]s by [[molecular weight]] of the fragments. RFMP is a [[Property|proprietary]] technology of [[GeneMatrix]] and can be utilized for [[genotyping]] [[viruses]] and [[microorganisms]], and for [[human genome]] research. |
'''Restriction Fragment Mass Polymorphism (RFMP)''' is a technology which [[Restriction digest|digests]] [[DNA]] into [[oligonucleotide]] fragments, and detects variation of [[DNA sequence]]s by [[molecular weight]] of the fragments. RFMP is a [[Property|proprietary]] technology of [[GeneMatrix]] and can be utilized for [[genotyping]] [[viruses]] and [[microorganisms]], and for [[human genome]] research. |
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== Overview == |
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Restriction fragment mass polymorphism (RFMP) is an application of [[Maldi-TOF|matrix-assisted laser desorption ionization time-of-flight]] (MALDI-TOF), used for identifying individual nucleotides from a DNA fragment, most commonly used in labeling [[Single-nucleotide polymorphism|single nucleotide polymorphisms]] (SNP). RFMP was developed as a successor to the similar [[restriction fragment length polymorphism]] (RFLP) with the intent to allow for more SNPs. Rather than read out lengths of fragments as RFLP does, the individual nucleotides are read out using MALDI-TOF, which gives specific clarity over same-length site cutting<ref>{{Cite journal|last=Hong|first=Sun Pyo|last2=Ji|first2=Seung Il|last3=Rhee|first3=Hwanseok|last4=Shin|first4=Soo Kyeong|last5=Hwang|first5=Sun Young|last6=Lee|first6=Seung Hwan|last7=Lee|first7=Soong Deok|last8=Oh|first8=Heung-Bum|last9=Yoo|first9=Wangdon|last10=Kim|first10=Soo-Ok|date=2008-06-09|title=A simple and accurate SNP scoring strategy based on typeIIS restriction endonuclease cleavage and matrix-assisted laser desorption/ionization mass spectrometry|url=https://pubmed.ncbi.nlm.nih.gov/18538037|journal=BMC genomics|volume=9|pages=276|doi=10.1186/1471-2164-9-276|issn=1471-2164|pmc=2442615|pmid=18538037}}</ref>. |
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== References == |
== References == |
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Revision as of 01:28, 19 November 2021
Restriction Fragment Mass Polymorphism (RFMP) is a technology which digests DNA into oligonucleotide fragments, and detects variation of DNA sequences by molecular weight of the fragments. RFMP is a proprietary technology of GeneMatrix and can be utilized for genotyping viruses and microorganisms, and for human genome research.
Overview
Restriction fragment mass polymorphism (RFMP) is an application of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF), used for identifying individual nucleotides from a DNA fragment, most commonly used in labeling single nucleotide polymorphisms (SNP). RFMP was developed as a successor to the similar restriction fragment length polymorphism (RFLP) with the intent to allow for more SNPs. Rather than read out lengths of fragments as RFLP does, the individual nucleotides are read out using MALDI-TOF, which gives specific clarity over same-length site cutting[1].
References
- Kim, Y (2005). "Population Genotyping of Hepatitis C Virus by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Short DNA Fragments", Clinical Chemistry, 51: 1123-1131
External links
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- ^ Hong, Sun Pyo; Ji, Seung Il; Rhee, Hwanseok; Shin, Soo Kyeong; Hwang, Sun Young; Lee, Seung Hwan; Lee, Soong Deok; Oh, Heung-Bum; Yoo, Wangdon; Kim, Soo-Ok (2008-06-09). "A simple and accurate SNP scoring strategy based on typeIIS restriction endonuclease cleavage and matrix-assisted laser desorption/ionization mass spectrometry". BMC genomics. 9: 276. doi:10.1186/1471-2164-9-276. ISSN 1471-2164. PMC 2442615. PMID 18538037.
{{cite journal}}: CS1 maint: unflagged free DOI (link)