Ellman's reagent: Difference between revisions
Content deleted Content added
TimVickers (talk | contribs) bold |
TimVickers (talk | contribs) refs |
||
| Line 1: | Line 1: | ||
'''Ellman's reagent''' - 5, 5'-dithiobis-(2-nitrobenzoic acid) or '''DTNB''' is a chemical used for measuring [[thiol]] groups.<ref> |
'''Ellman's reagent''' - 5, 5'-dithiobis-(2-nitrobenzoic acid) or '''DTNB''' is a chemical used for measuring [[thiol]] groups.<ref>{{cite journal |author=Boyne AF, Ellman GL |title=A methodology for analysis of tissue sulfhydryl components |journal=Anal. Biochem. |volume=46 |issue=2 |pages=639-53 |year=1972 |pmid=4623507}}</ref> The compound reacts with a thiol group to release a 2-nitro-5-mercaptobenzoic acid (TNB<sup>-</sup>), which has a strong yellow color in water at neutral and alkaline pH. The TNB<sup>-</sup> is quantified by measuring its absorbance of visible light at 412 nm using a absorbance coefficient of 14.14mM cm<sup>-1</sup>.<ref>{{cite journal |author=Collier HB |title=Letter: A note on the molar absorptivity of reduced Ellman's reagent, 3-carboxylato-4-nitrothiophenolate |journal=Anal. Biochem. |volume=56 |issue=1 |pages=310-1 |year=1973 |pmid=4764694}}</ref> Ellman's reagent can be used for measuring both low-molecular mass thiols such as [[glutathione]] as well as thiol groups on proteins.<ref>{{cite journal |author=Riener CK, Kada G, Gruber HJ |title=Quick measurement of protein sulfhydryls with Ellman's reagent and with 4,4'-dithiodipyridine |journal=Analytical and bioanalytical chemistry |volume=373 |issue=4-5 |pages=266-76 |year=2002 |pmid=12110978}}</ref> |
||
==References== |
==References== |
||
Revision as of 00:09, 7 September 2007
Ellman's reagent - 5, 5'-dithiobis-(2-nitrobenzoic acid) or DTNB is a chemical used for measuring thiol groups.[1] The compound reacts with a thiol group to release a 2-nitro-5-mercaptobenzoic acid (TNB-), which has a strong yellow color in water at neutral and alkaline pH. The TNB- is quantified by measuring its absorbance of visible light at 412 nm using a absorbance coefficient of 14.14mM cm-1.[2] Ellman's reagent can be used for measuring both low-molecular mass thiols such as glutathione as well as thiol groups on proteins.[3]
References
- ^ Boyne AF, Ellman GL (1972). "A methodology for analysis of tissue sulfhydryl components". Anal. Biochem. 46 (2): 639–53. PMID 4623507.
- ^ Collier HB (1973). "Letter: A note on the molar absorptivity of reduced Ellman's reagent, 3-carboxylato-4-nitrothiophenolate". Anal. Biochem. 56 (1): 310–1. PMID 4764694.
- ^ Riener CK, Kada G, Gruber HJ (2002). "Quick measurement of protein sulfhydryls with Ellman's reagent and with 4,4'-dithiodipyridine". Analytical and bioanalytical chemistry. 373 (4–5): 266–76. PMID 12110978.
{{cite journal}}: CS1 maint: multiple names: authors list (link)