P1 phage: Difference between revisions
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'''P1''' is a [[Temperateness_(virology)|temperate]] [[bacteriophage]] (phage) that infects ''[[Escherichia coli]]'' and a some other bacteria. When undergoing a [[lysogenic cycle]] the phage genome exists as a [[plasmid]] in the bacterium.<ref name=Lobocka2004>{{Cite journal| doi = 10.1128/JB.186.21.7032-7068.2004| issn = 0021-9193| volume = 186| issue = 21| pages = 7032-7068| last = Łobocka| first = Małgorzata B.| coauthors = Debra J. Rose, Guy Plunkett, Marek Rusin, Arkadiusz Samojedny, Hansjörg Lehnherr, Michael B. Yarmolinsky, Frederick R. Blattner| title = Genome of Bacteriophage P1| journal = Journal of Bacteriology| date = 2004-11| pmc=523184|url=http://jb.asm.org/content/186/21/7032}}</ref> |
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'''P1''' is a temperate [[bacteriophage]] (phage). The P1 phage can be used to create the [[P1-derived artificial chromosome]] [[cloning vector]]. |
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The P1 phage has gained research interest because it can be used to create the [[P1-derived artificial chromosome]] [[cloning vector]] which can carry relatively large fragments of DNA. Also, P1 encodes a site-specific recombinase, Cre, that is widely used to carry out cell-specific or time-specific DNA recombination by flanking the target DNA with ''loxP'' sites.(see [[Cre-Lox recombination]]) |
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==Life cycle== |
==Life cycle== |
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A unique feature of phage P1 is that during lysogeny its genome is not incorporated into the bacterial chromosome as is commonly observed during lysogeny of other bacteriophage. Instead, P1 exists independently within the bacterial cell, much like a [[plasmid]] would. P1 replicates as a 90 [[Base pair|kilobase]] (kb) plasmid in the lysogenic state and is partitioned equally into two new daughter cells during normal [[cell division]]. |
A unique feature of phage P1 is that during lysogeny its genome is not incorporated into the bacterial chromosome as is commonly observed during lysogeny of other bacteriophage. Instead, P1 exists independently within the bacterial cell, much like a [[plasmid]] would. P1 replicates as a 90 [[Base pair|kilobase]] (kb) plasmid in the lysogenic state and is partitioned equally into two new daughter cells during normal [[cell division]]. |
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P1 encodes a site-specific recombinase, Cre, that is widely used to promote cell-specific or time-specific DNA recombination via flanking ''loxP'' sites |
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==References== |
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{{Reflist}} |
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==External links== |
==External links== |
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* [http://www.expasy.org/viralzone/all_by_species/505.html '''Viralzone''': P1-like phage] |
* [http://www.expasy.org/viralzone/all_by_species/505.html '''Viralzone''': P1-like phage] |
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{{Microbiology-stub}} |
{{Microbiology-stub}} |
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Revision as of 06:19, 26 March 2012
| P1 phage | |
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| Virus classification | |
| Group: | Group I (dsDNA)
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| Species: | P1 Phage
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P1 is a temperate bacteriophage (phage) that infects Escherichia coli and a some other bacteria. When undergoing a lysogenic cycle the phage genome exists as a plasmid in the bacterium.[1]
The P1 phage has gained research interest because it can be used to create the P1-derived artificial chromosome cloning vector which can carry relatively large fragments of DNA. Also, P1 encodes a site-specific recombinase, Cre, that is widely used to carry out cell-specific or time-specific DNA recombination by flanking the target DNA with loxP sites.(see Cre-Lox recombination)
Life cycle
Temperate phage, such as P1, have the ability to exist within the bacterial cell they infect in two different ways. In lysogeny, P1 can exist within a bacterial cell as a circular DNA in that it exists by replicating as if it were a plasmid and does not cause cell death. Alternatively, in its lytic phase, P1 can promote cell lysis during growth resulting in host cell death. During lysogeny new phage particles are not produced. In contrast, during lytic growth many new phage particles are assembled and released from the cell. By alternating between these two modes of infection, P1 can survive during extreme nutritional conditions that may be imposed upon the bacterial host in which it exists.
A unique feature of phage P1 is that during lysogeny its genome is not incorporated into the bacterial chromosome as is commonly observed during lysogeny of other bacteriophage. Instead, P1 exists independently within the bacterial cell, much like a plasmid would. P1 replicates as a 90 kilobase (kb) plasmid in the lysogenic state and is partitioned equally into two new daughter cells during normal cell division.
References
- ^ Łobocka, Małgorzata B. (2004-11). "Genome of Bacteriophage P1". Journal of Bacteriology. 186 (21): 7032–7068. doi:10.1128/JB.186.21.7032-7068.2004. ISSN 0021-9193. PMC 523184.
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External links
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