Plaque hybridization: Difference between revisions
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Revision as of 08:11, 4 July 2015
Plaque hybridization is a technique used in Molecular biology for the identification of recombinant phages.[1] The procedure can also be used for the detection of differentially represented repetitive DNA. The technique involves the hybridization of isolated DNA from each of the thousands of the phage λ, from our cDNA to a label probe for our gene of study. This is followed by autoradiography to detect the position of the label.[2] The plaque hybridization procedure is similar to Colony hybridization but have some advantages over the later due to the smaller and well defined area of the filter to which the DNA bounds.[3]
References
- ^ Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 21374207, please use {{cite journal}} with
|pmid=21374207instead. - ^ Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 7727126, please use {{cite journal}} with
|pmid=7727126instead. - ^ "Nylon Membranes for Colony and Plaque Hybridization". Life science. Retrieved June 4, 2015.