NgAgo: Difference between revisions
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==Controversy== |
==Controversy== |
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There have recently been several allegations that this procedure is impossible to reproduce. Nature Biotechnology, which originally published the research, is investigating. |
There have recently been several allegations that this procedure is impossible to reproduce. Nature Biotechnology, which originally published the research, is investigating. |
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<ref>{{cite web|last1=Cyranoski|first1=David|title=Replications, ridicule and a recluse: the controversy over NgAgo gene-editing intensifies|url=http://www.nature.com/news/replications-ridicule-and-a-recluse-the-controversy-over-ngago-gene-editing-intensifies-1.20387?WT.mc_id=SFB_NNEWS_1508_RHBox|website=nature.com|accessdate=August 17, 2016}}</ref> |
<ref>{{cite web|last1=Cyranoski|first1=David|title=Replications, ridicule and a recluse: the controversy over NgAgo gene-editing intensifies|url=http://www.nature.com/news/replications-ridicule-and-a-recluse-the-controversy-over-ngago-gene-editing-intensifies-1.20387?WT.mc_id=SFB_NNEWS_1508_RHBox|website=nature.com|accessdate=August 17, 2016}}</ref><ref>{{cite web|last1=Cyranoski|first1=David|title=NgAgo gene-editing controversy escalates in peer-reviewed papers|doi=10.1038/nature.2016.21023 |
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|website=nature.com|accessdate=November 25, 2016|url=http://www.nature.com/news/ngago-gene-editing-controversy-escalates-in-peer-reviewed-papers-1.21023?WT.mc_id=SFB_NNEWS_1508_RHBox}}</ref>10.1038/nature.2016.21023 |
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==References== |
==References== |
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{{Reflist}} |
{{Reflist}} |
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Revision as of 12:19, 25 November 2016
NgAgo is an acronym for Natronobacterium gregoryi Argonaute. NgAgo is a single-stranded DNA (ssDNA)-guided Argonaute endonuclease. NgAgo binds 5′ phosphorylated ssDNA of ~24 nucleotides (gDNA) to guide it to its target site and will make DNA double-strand breaks at gDNA site. In contrast to Cas9, the NgAgo–gDNA system does not require a protospacer adjacent motif (PAM). Like the Crispr/Cas system the NgAgo is suitable for genome editing.[1]
Role
NgAgo is useful for genome editing because of the system’s high accuracy and efficiency which minimizes off-target effects. The specificity of the gDNA is essential, as cleavage efficiency is impaired by a single nucleotide mismatch between the guide and target molecules. Using 5’ phosphorylated ssDNAs as guide molecules reduces the possibility of cellular oligonucleotides misleading NgAgo. A guide molecule can only be attached to NgAgo during the expression of the protein. Once the guide is loaded, NgAgo cannot swap free floating ssDNA for its gDNA. Designing, synthesizing, and adjusting the concentration of ssDNAs is easier compared to systems using sgRNA. The required dosage of ssDNA is less than that of a sgRNA expression plasmid.[2]
Controversy
There have recently been several allegations that this procedure is impossible to reproduce. Nature Biotechnology, which originally published the research, is investigating. [3][4]10.1038/nature.2016.21023
References
- ^ Gao, Feng, et al. "DNA-guided genome editing using the Natronobacterium gregoryi Argonaute." Nature biotechnology (2016).
- ^ Cato, Tim. "DNA-guided genome editing using the Natronobacterium gregoryi Argonaute win". nature.com. Retrieved June 20, 2016.
- ^ Cyranoski, David. "Replications, ridicule and a recluse: the controversy over NgAgo gene-editing intensifies". nature.com. Retrieved August 17, 2016.
- ^ Cyranoski, David. "NgAgo gene-editing controversy escalates in peer-reviewed papers". nature.com. doi:10.1038/nature.2016.21023. Retrieved November 25, 2016.
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