Zoo blot: Difference between revisions
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A '''zoo blot''' or '''garden blot''' is a type of [[Southern blot]] that demonstrates the similarity between specific, usually protein-coding, [[DNA sequence]]s of different species. A zoo blot compares animal species while a garden blot compares plant species. The purpose of the zoo blot is to detect the conservation of the gene(s) of interest throughout the evolution of different species.<ref>{{cite book|title=Biotechnology Fundamentals|first1=Firdos Alam |last1=Khan |publisher=CRC Press|date=20 September 2011|url=https://books.google.com/books?id=-s5oRDUuMSIC&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false|page=563}}</ref> |
A '''zoo blot''' or '''garden blot''' is a type of [[Southern blot]] that demonstrates the similarity between specific, usually protein-coding, [[DNA sequence]]s of different species. A zoo blot compares animal species while a garden blot compares plant species. The purpose of the zoo blot is to detect the conservation of the gene(s) of interest throughout the evolution of different species.<ref>{{cite book|title=Biotechnology Fundamentals|first1=Firdos Alam |last1=Khan |publisher=CRC Press|date=20 September 2011|url=https://books.google.com/books?id=-s5oRDUuMSIC&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false|page=563}}</ref> |
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In order to understand the degree to which a particular gene is similar from species to species, DNA preparations from a set of species is isolated and spread over a surface. The sequence of interest is labeled and allowed to hybridize to the prepared DNA. Usually, the labeled DNA is marked with a [[phosphorus#Isotopes|radioactive isotope of phosphorus]]. The hybridization is a process that happens spontaneously: DNA pairs with complementary strands. The hybridization, however, is not perfect. |
In order to understand the degree to which a particular gene is similar from species to species, DNA preparations from a set of species is isolated and spread over a surface. The sequence of interest is labeled and allowed to hybridize to the prepared DNA. Usually, the labeled DNA is marked with a [[phosphorus#Isotopes|radioactive isotope of phosphorus]]. The hybridization is a process that happens spontaneously: DNA pairs with complementary strands. The hybridization, however, is not perfect.<ref>{{cite book|title=Molecular Biomethods Handbook |url=https://moodle.ufsc.br/pluginfile.php/1376620/mod_resource/content/0/7170_Molecular%20Biomethods.pdf|first1=John|last1=Walker|display-authors=etal|publisher=Humana Press|date=2008|doi= 10.1007/978-1-60327-375-6|page=8}}</ref> |
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The hybridization of two strands will happen even when the strands are similar but not identical. This procedure is used to detect similar or exact relationships between the DNA in question and other organisms, so the technique takes advantage of non-exact hybridization. It also allows you judge the locations of introns and exons as the latter will be far more conserved than the former. |
The hybridization of two strands will happen even when the strands are similar but not identical. This procedure is used to detect similar or exact relationships between the DNA in question and other organisms, so the technique takes advantage of non-exact hybridization. It also allows you judge the locations of introns and exons as the latter will be far more conserved than the former. |
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Revision as of 21:13, 19 November 2017
 | This article needs additional citations for verification. (November 2017) |
A zoo blot or garden blot is a type of Southern blot that demonstrates the similarity between specific, usually protein-coding, DNA sequences of different species. A zoo blot compares animal species while a garden blot compares plant species. The purpose of the zoo blot is to detect the conservation of the gene(s) of interest throughout the evolution of different species.[1]
In order to understand the degree to which a particular gene is similar from species to species, DNA preparations from a set of species is isolated and spread over a surface. The sequence of interest is labeled and allowed to hybridize to the prepared DNA. Usually, the labeled DNA is marked with a radioactive isotope of phosphorus. The hybridization is a process that happens spontaneously: DNA pairs with complementary strands. The hybridization, however, is not perfect.[2]
The hybridization of two strands will happen even when the strands are similar but not identical. This procedure is used to detect similar or exact relationships between the DNA in question and other organisms, so the technique takes advantage of non-exact hybridization. It also allows you judge the locations of introns and exons as the latter will be far more conserved than the former.
See also
This relies on the conservation of DNA exon sequences between species, hence the name. The theory is that sequences conserved between species are more likely to represent expressed genes or coding sequences. The technique used here is similar to cDNA selection except the cDNA probe is replaced with genomic DNA isolated from different species.
References
- ^ Khan, Firdos Alam (20 September 2011). Biotechnology Fundamentals. CRC Press. p. 563.
- ^ Walker, John; et al. (2008). Molecular Biomethods Handbook (PDF). Humana Press. p. 8. doi:10.1007/978-1-60327-375-6.
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